Optimal design of a four‐zone simulated moving bed process for separation of homoharringtonine and harringtonine

Kim, Seul-Gi; Nam, Hee-Geun; Kim, Jin-Hyun; Mun, Sungyong

doi:10.1002/cjce.20401

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…In addition, HHT is inexpensive and easy to produce. HHT is extracted from Cephalotaxaceae conifers, which are widely distributed in subtropical, evergreen, and broad-leaved forests [43]. The HHT extraction process is simple.…”

Section: Discussionmentioning

confidence: 99%

TMEM16A, a Homoharringtonine Receptor, as a Potential Endogenic Target for Lung Cancer Treatment

Guo

Bai

Shi

et al. 2021

IJMS

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Lung cancer has the highest rate of incidence and mortality among all cancers. Most chemotherapeutic drugs used to treat lung cancer cause serious side effects and are susceptible to drug resistance. Therefore, exploring novel therapeutic targets for lung cancer is important. In this study, we evaluated the potential of TMEM16A as a drug target for lung cancer. Homoharringtonine (HHT) was identified as a novel natural product inhibitor of TMEM16A. Patch-clamp experiments showed that HHT inhibited TMEM16A activity in a concentration-dependent manner. HHT significantly inhibited the proliferation and migration of lung cancer cells with high TMEM16A expression but did not affect the growth of normal lung cells in the absence of TMEM16A expression. In vivo experiments showed that HHT inhibited the growth of lung tumors in mice and did not reduce their body weight. Finally, the molecular mechanism through which HHT inhibits lung cancer was explored by western blotting. The findings showed that HHT has the potential to regulate TMEM16A activity both in vitro and in vivo and could be a new lead compound for the development of anti-lung-cancer drugs.

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Section: Discussionmentioning

confidence: 99%

TMEM16A, a Homoharringtonine Receptor, as a Potential Endogenic Target for Lung Cancer Treatment

Guo

Bai

Shi

et al. 2021

IJMS

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“…pruniflorum (Kurz) Gogelein also had, at least a similarly high level of anti-HIV-1 RT activity as the standard drug. The alkaloids extract from Phyllanthus niruri could decrease cytopathogenicity in MT-4 cells caused by HIV infection (Kim et al, 2011) and Camellia sinensis extract, which contain epicatechin gallate and other substances, expressed inhibitory effects on the HIV RT enzyme using radioactive assay (Nagao et al, 1996). In addition, the metabolites could also be used as natural precursors for the semi-synthesis of high potential compounds and this modification can also improve the already proven high levels of bioactivity, such as anti-viral activity, of several basic structures (Venditti et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Isolation of stigmasterol from Kra Don (Careya arborea Roxb.) and bioactivities of its crude extracts against free radicals and human immunodeficiency virus

2021

ANRES

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Careya arborea Roxb. (family Lecythidaceae) has long been used as a medicinal plant for the treatment of many diseases. In this study, extracts were taken from the stem bark, wood and leaves using methanol and then investigated for antioxidant activities, such as 2,2-diphenyl-1picrylhydrazyl free radical scavenging activity, nitric oxide radical scavenging activity (NO), superoxide radical scavenging activity (SOD), ferric reducing antioxidant power (FRAP), total phenolic content (TPC) and total flavonoid content (TFC). For the antioxidant activities, the stem bark extract had the highest mean (±SD) levels (623.96 ± 12.50 µg/mL, 140.86 ± 2.63 µg/mL, 152.38 ± 6.35 µg/mL, 252.44 ± 4.87 mg Trolox equivalent/g and 128.19 ± 2.12 mg gallic acid equivalent/g, respectively) except for the total flavonoid content, for which the leaf extract produced the highest amount (30.62 ± 0.29 mg quercetin equivalent/g). However, regarding the anti-human immunodeficiency virus (HIV) activity using an HIV-1 reverse transcriptase inhibition assay, the stem bark and leaf extracts had a high inhibitory effect against HIV-1 reverse transcriptase with values of more than 100% of the relative inhibition for a comparable level of the standard drug (Nevirapine). The highest ranking of the antioxidant and anti-HIV activities of the fractions was the stem bark followed by the leaf and wood extracts, respectively. Stigmasterol was isolated from combined fraction number 3 of the lipophilic extract eluted from n-hexane-ethyl acetate (95:5) via column chromatography. The 1 H and 13 C NMR data obtained were in accordance with previous reports. Future study will investigate further isolation of these bioactive compounds and evaluation of their bioactivities.

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Preparative Chromatography: Batch and Continuous

Aniceto

Silva

2015

Analytical Separation Science

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Chromatography, although initially prevalent in the analytical field, is increasingly extended as a preparative process for the commercial scale production of several chemicals whose separation from feed streams is difficult to achieve by conventional methods. In this chapter, the most relevant and recent preparative chromatography approaches are presented and discussed, divided in terms of batch and continuous processes. The fundamental conservation equations, kinetic laws, and equilibrium relations governing chromatographic systems are presented in detail as they are common to all processes modeling. Several models and approaches are presented in an order of higher approximation to reality that necessarily results in an increasing degree of mathematical sophistication. This complexity trend is linked to the sequential introduction of the various phenomena that take place inside the adsorption bed. In particular, the influence of finite mass transfer resistances and axial dispersion upon the dynamic behavior of the chromatographic system is highlighted. With regard to batch chromatography, the most frequent techniques, such as preparative HPLC and supercritical fluid chromatography are covered, along with key chromatography concepts. Closed loop recycling chromatography and steady‐state recycling chromatography are recycling techniques that may be situated between batch and continuous strategies, being able to enhance the performance of batch systems while avoiding the higher complexity inherent to continuous approaches. Regarding continuous chromatography, the development of the simulated moving bed ( SMB ) technology has allowed improved productivity, product's purities, and lower operating costs compared to batch processing. Since its inception, several different strategies and modifications to the conventional SMB have been proposed. The well‐established and the most innovative SMB operation modes, such as intermittent‐ SMB , supercritical SMB , and variable external streams systems, are presented and discussed, along with the necessary equipment designs that allow their implementation. Other continuous chromatographic processes include the high‐speed countercurrent chromatography and the centrifugal partition chromatography, both involving two immiscible liquid phases, and annular chromatography that permits continuous operation while using a stationary phase. Some brief remarks are made on equipment and system designs.

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Optimal design of a four‐zone simulated moving bed process for separation of homoharringtonine and harringtonine

Cited by 5 publications

References 16 publications

TMEM16A, a Homoharringtonine Receptor, as a Potential Endogenic Target for Lung Cancer Treatment

TMEM16A, a Homoharringtonine Receptor, as a Potential Endogenic Target for Lung Cancer Treatment

Isolation of stigmasterol from Kra Don (Careya arborea Roxb.) and bioactivities of its crude extracts against free radicals and human immunodeficiency virus

Preparative Chromatography: Batch and Continuous

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