A new type of bismuth doped Ba(2)B(5)O(9)Cl crystal is reported to exhibit broadband near infrared (NIR) photoluminescence at room temperature, which has been identified here originating from elementary bismuth atom. Rietveld refining, static and dynamic spectroscopic properties reveal two types of Bi(0) centers in the doped compound due to the successful substitution for two different nine-coordinated barium lattice sites. These centers can be created only in a reducing condition, and when treated in air and N(2)/H(2) flow in turn, they can be removed and restored reversely. As the dwelling time is prolonged in N(2)/H(2) at high temperature, conversion from Bi(2+) to Bi(0), as reflected by changes of their relative emission intensities, is witnessed in the crystal of Ba(2)B(5)O(9)Cl:Bi. The lifetime of the NIR luminescence was observed in a magnitude of ~30 μs, rather different from bismuth doped either glasses or crystals reported previously.

CD58 and CD2 have long been known as a pair of reciprocal adhesion molecules involved in the immune modulations of CD8+ T and NK-mediated cellular immunity in humans and several other mammals. However, the functional roles of CD58 and CD2 in CD4+ T-mediated adaptive humoral immunity remain poorly defined. Moreover, the current functional observations of CD58 and CD2 were mainly acquired from in vitro assays, and in vivo investigation is greatly limited due to the absence of a Cd58 homology in murine models. In this study, we identified cd58 and cd2 homologs from the model species zebrafish (Danio rerio). These two molecules share conserved structural features to their mammalian counterparts. Functionally, cd58 and cd2 were significantly upregulated on antigen-presenting cells and Cd4+ T cells upon antigen stimulation. Blockade or knockdown of Cd58 and Cd2 dramatically impaired the activation of antigen-specific Cd4+ T and mIgM+ B cells, followed by the inhibition of antibody production and host defense against bacterial infections. These results indicate that CD58/CD2 interaction was required for the full activation of CD4+ T-mediated adaptive humoral immunity. The interaction of Cd58 with Cd2 was confirmed by co-immunoprecipitation and functional competitive assays by introducing a soluble Cd2 protein. This study highlights a new costimulatory mechanism underlying the regulatory network of adaptive immunity and makes zebrafish an attractive model organism for the investigation of CD58/CD2-mediated immunology and disorders. It also provides a cross-species understanding of the evolutionary history of costimulatory signals from fish to mammals as a whole.

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Bismuth-doped glasses have received much attention in the last decade due to their broadband nearinfrared (NIR) emission. Their optical properties are sensitive to composition and there are a few reports that qualitatively describe the dependence of these properties on their compositions. Yet the actual role of bismuth, as the matrix composition changes, remains fundamentally unclear. In this work, we investigate optical properties of bismuth-doped germanate glasses with different compositions and interpret their dependences in terms of their microscopic structure using Raman and Fourier transform infrared (FTIR) spectra. Beyond qualitative descriptions, quantitative empirical prediction laws are established on the grounds of the relationships between compositions, structures and properties of bismuth-doped germanate glasses. These findings enable precise predictions of the frequency shift of NIR luminescence from bismuth-doped glasses and a better understanding of the nature of bismuth NIR luminescent centers.

Our subject is an electromagnetic field near the plane interface dividing a conductive and a dielectric media, under conditions supporting the surface plasmon-polariton (SPP) propagation. The conductive medium is described by the hydrodynamic electron-gas model which enables a consistent analysis of the field-induced variations of the electron density and velocity at the interface and its nearest vicinity. The distributions of electromagnetic dynamical characteristics: energy, energy flow, spin and momentum are calculated analytically and illustrated numerically, employing the silver-vacuum interface as an example. A set of the "field" and material contributions to the energy, spin and momentum are explicitly identified in respect to their physical origins, and the orbital (canonical) and spin (Belinfante) momentum constituents are separately examined. In this context, a procedure for the spin-orbital momentum decomposition in presence of free charges is proposed and substantiated. The microscopic results agree with the known phenomenological data but additionally show specific nanoscale structures in the near-interface behavior of the SPP energy and momentum which can be deliberately created, controlled and used in nanotechnology applications.

The nature of bismuth NIR luminescence is essential to develop the bismuth doped laser materials with high efficiency and desirable emission wavelength, and it, thereby, receives rising interests. Our previous work reported the Bi(0) luminescence from Ba2B5O9Cl: Bi with a lifetime of ~30 μs and the conversion of Bi(2+) to Bi(0). This work found indeed the conversion could be enabled in the compound by an in situ reduction technique and it, however, happens via an intermediate state of Bi(+). Once the ion of Bi(+) is stabilized and built into the compound, it can luminesce in a super broad spectral range from 600 to 1200 nm with a lifetime longer than 1 ms, due to the cascade transitions from (3)P2 and (3)P1 to (3)P0. This is completely different from Bi(0) and Bi(2+) in the compound, and it has never been noticed before. We believe this work can help us better understand the complex nature of bismuth luminescence.

Rapid, accurate, and safe screening of foodborne pathogenic bacteria is essential to effectively control and prevent outbreaks of foodborne illness. Fluorescent sensors constructed from carbon dots (CDs) and nanomaterial-based quenchers have provided an innovative method for screening of pathogenic bacteria. Herein, an ultrasensitive magnetic fluorescence aptasensor was designed for separation and detection of Staphylococcus aureus (S. aureus). Multicolor fluorescent CDs with a long fluorescent lifetime (6.73 ns) and high fluorescence stability were synthesized using a facile hydrothermal approach and modified cDNA as a highly sensitive fluorescent probe. CD fluorescence was quenched by Fe3O4 + aptamer via fluorescence resonance energy transfer (FRET). Under optimal conditions, the FRET-based aptasensor can detect S. aureus accompanied by a wide linear range of 50–107 CFU·mL–1 and a detection limit of 8 CFU·mL–1. Compared with other standard methods, this method was faster and more convenient, and the entire test was finished within 30 min. The capability of the aptasensor was simultaneously investigated on food samples. Additionally, the developed CDs exhibited excellent biocompatibility and were thus applied as fluorescent probes for bioimaging both in vitro and in vivo. This new platform provided an excellent application of the CDs for detecting and bioimaging pathogenic bacteria.

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