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The aim of this study was to evaluate the oxidant profile and iron metabolism in serum of dogs infected by Ehrlichia canis. Banked sera samples of dogs were divided into two groups: negative control (n = 17) and infected by E. canis on acute (n = 24), and subclinical (n = 18) phases of the disease. The eritrogram, leucogram, and platelet counts were evaluate as well as iron, ferritin, and transferrin levels, latent iron binding capacity (LIBC), and transferrin saturation index (TSI) concentration. In addition, the advanced oxidation protein products (AOPP) and ferric reducing ability of plasma (FRAP) in sera were also analyzed. Blood samples were examined for the presence of E. canis by PCR techniques. History and clinical signals were recorded for each dog. During the acute phase of the disease, infected animals showed thrombocytopenia and anemia when compared to healthy animals (P < 0.05) as a consequence of lower iron levels. Ferritin and transferrin levels were higher in both phases (acute and subclinical) of the disease. The AOPP and FRAP levels increased in infected animals on the acute phase; however, the opposite occurred in the subclinical phase. We concluded that dogs naturally infected by E. canis showed changes in the iron metabolism and developed an oxidant status in consequence of disease pathophysiology.

The objective of this study was to assess the association among sexual maturation (SM), overweight and central adiposity in children and adolescents. A total of 617 children and adolescents age 8 to 18 years old participated in the longitudinal study. Three samples were collected including data on weight, height and waist circumference. Overweight was classi-fied based on critical values for body mass index (BMI) adopted for Brazilian children and teenagers. SM stage (SMS) was done by self-assessment in three children and adolescent samples. Participants were distributed in quartiles based on SMS and sex. Accelerated-maturing subjects were compared to others. Data was analyzed by linear regression and logistic regression. We found a negative association between BMI z-score and onset of SM in boys, whereas for girls relatively-accelerated SM was positively associated to overweight and values of BMI z-score. Relatively-accelerated maturing girls showed more central adi-posity. The research concluded that accelerated SM was associated to overweight and high-er increase in BMI for both sexes, highlighting the importance of identifying SMS in nutri-tional assessment for children and teenagers.

Background: Hemostatic alterations are commonly detected in canine cancer patients. However, few studies have described hemostatic dysfunction in dogs with different tumor subtypes. In Veterinary Medicine, the state of hypercoagulability is hardly diagnosed alive, since laboratory exams for evaluate hemostatic function are not always requested. Due to importance of homeostatic disorders in cancer patients, this study aimed to evaluate hemostatic alterations such as platelet count, activated partial thromboplastin time (aPTT), prothrombin time (PT) and fibrinogen in tumor-bearing dogs.Materials, Methods & Results: From the 55 dogs evaluated, 30 had mammary carcinoma, 6 visceral hemangiosarcoma, 9 high-grade cutaneous mast cell tumor and 10 multicentric lymphoma. The results were compared to a control group composed by 10 Beagle dogs. Thrombocytosis was observed in 26.6% (8/30) of mammary carcinoma group and thrombocytopenia in 10% (3/30). The patients with hemangiosarcoma and mast cell tumor did not reveal thrombocytosis, however, thrombocytopenia was present in 16.6% (1/6) and 33% (3/9), respectively. Three dogs with multicentric lymphoma showed thrombocytopenia and other three showed thrombocytosis. From patients with thrombocytosis, one was classified as severe thrombocytosis (1077 x 10³/µL). Therefore, there were no statistically significant associations between neoplasia group with control group (P > 0.05). Regarding the aPTT and PT evaluation, mammary carcinoma (P = 0.0005), hemangiosarcoma (P = 0.033) and mast cell tumor (P = 0.012) patients showed statistical difference for aPTT, while the evaluation for PT was not significant (P > 0.05). We grouped all patients as a “tumor group” and compared to the control group. It was possible to observe increased aPTT and PT in 89% (49/55) and 50.90% (28/55) respectively, in tumor group compared to normal. A total of 47.27% (n = 26) of the patients with tumors presented increased aPTT and PT concomitantly. In the present study, 14.54% of the patients presented elevated levels of fibrinogen associated with increased aPTT. However, only the mast cell tumor group was statistically significant (P = 0.043).Discussion: Hemostatic alterations can be found in dogs with cancer and when these alterations occurs, can be directly associated with tumoral non-invasive actions called as paraneoplastic syndrome. However, the hemostatic paraneoplastic syndrome is poorly reported in veterinary medicine, with limited number of papers describing this condition. Our results indicated that the presence of thrombocytosis in patients with tumors could be related with the production of granulocytemacrophage colony stimulating factors (GM-CSF) and IL-6 by tumor cells. A total of 26 patients with tumors presented increased aPTT and PT concomitantly, confirming that hemostatic dysfunction is a common alteration in dogs with neoplasia. However, despite alterations in coagulation parameters, there were no clinical manifestations of bleeding such as petechial or bruising in these patients. The increased fibrinogen and aPTT can be caused by a systemic inflammatory reaction mediated by pro-inflammatory cytokines produced by tumors cells. Based on that, 14.54% of the animals presented elevated levels of fibrinogen associated with elevated aPTT suggesting that these patients are associated with systemic inflammation and tumor progression. This study suggested that bearing-tumors patients shows important hemostatic dysfunctions, elucidating the clinical importance of these results in veterinary medicine.