Iron metabolism and oxidative profile of dogs naturally infected by Ehrlichia canis : Acute and subclinical disease

Bottari, Nathieli B.; Crivellenti, Leandro Zuccolotto; Borin-Crivellenti, Sofia; Oliveira, Jéssica Rodrigues de; Coelho, Stefanie Bertti; Contin, Catarina Micali; Tatsch, Etiane; Moresco, Rafael Noal; Santana, Áureo Evangelista; Tonin, Alexandre A.; Tinucci‐Costa, Mirela; Silva, Aleksandro S. Da

doi:10.1016/j.micpath.2015.11.030

Cited by 11 publications

(10 citation statements)

References 31 publications

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“…As antioxidant biomarkers, three assays commonly used to evaluate the total antioxidant capacity (TAC) were included in this study: trolox equivalent antioxidant capacity (TEAC), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP), as well as two individual antioxidants, uric acid and thiol [20][21][22]. Regarding oxidant status, it was evaluated using assays for ferrous oxidation-xylenol orange (FOX) levels, total oxidant content (TOS), advanced oxidation protein products (AOPP) and hydrogen peroxide (H 2 O 2 ) [23].…”

Section: Introductionmentioning

confidence: 99%

Changes in Serum Biomarkers of Oxidative Stress in Cattle Vaccinated with Tick Recombinant Antigens: A Pilot Study

et al. 2020

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Tick vaccination is an environmentally friendly alternative for tick control, pathogen infection, and transmission. Tick vaccine protection is sometimes incomplete, which may be due to problems in the stability, conformation, and activity of antibodies. This might be related to oxidative stress, but more studies are needed about the possible relationships between oxidative stress and immune function. The objective of this study was to evaluate and compare various serum biomarkers of antioxidant response and oxidative damage in cattle vaccinated with two recombinant antigens, the chimera of Subolesin- BM95 (homologue antigen of BM86)-MSP1a and BM86, and a control consisting in the adjuvant of the vaccines. Cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of the plasma (FRAP), trolox equivalent antioxidant capacity (TEAC), total thiol concentrations, and uric acid were evaluated in serum to determine the antioxidant response. To evaluate oxidative status, ferrous oxidation-xylenol orange (FOX), total oxidant status (TOS), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) concentrations in serum were determined. In addition, correlations between biomarkers of oxidative stress and antibody titers were evaluated. A significant decrease in all antioxidant biomarkers, with exception of thiol, and also a decrease in the oxidant markers TOS, AOPP and H2O2 was observed in cattle vaccinated with BM86, that also showed the highest antibody titers response whereas no significant differences in any of the biomarkers were detected in the Subolesin-Bm95-MSP1a and control groups. In addition, the dynamics of Cuprac and H2O2 with time showed significant differences between the groups. Although this is a pilot study and the results should be interpreted with caution and corroborated by studies involving a large number of animals, our results indicate that, in our experimental conditions, those vaccines able to induce a lower oxidative stress produce a higher concentration of antigen-specific antibodies. Overall, the results of the study provided information on the behavior of different biomarkers related to antioxidant defense, and the oxidative damage in cattle in response to vaccination.

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Section: Introductionmentioning

confidence: 99%

Changes in Serum Biomarkers of Oxidative Stress in Cattle Vaccinated with Tick Recombinant Antigens: A Pilot Study

et al. 2020

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“…Thrombocytopenia, anemia and lymphopenia were also related to the action of Ehrlichia in the bone marrow and lymphoid tissues (Mylonakis et al 2004, Greene 2006. Recent studies about iron metabolism in animals infected with Ehrlichia canis, reported a marked decreased in circulating iron, suggesting that the host may be induced by the bacteria to transport iron to infected tissue for bacterial multiplication, contributing to anemia (Bottari et al 2016). This may explain the numerous hemosiderin granules commonly found in the bone marrow, liver, lymph nodes and spleen from animals with ehrlichiosis in this study.…”

Section: Discussionmentioning

confidence: 99%

Differential diagnoses in 83 dogs with icterus

et al. 2020

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Icterus (jaundice) is a yellowish pigmentation resulting from the depositing of bilirubin in tissues due to its high plasmatic concentration. The pathogenesis of icterus includes metabolic changes or obstructed bilirubin excretion and it is classified as pre-hepatic, hepatic and post-hepatic. This study aimed to evaluate and classify different causes of icterus in dogs during post mortem examination. These dogs were examined from 2014 to 2017, using macroscopic and histologic exams as well as ancillary tests. Eighty-three dogs were examined macroscopically and microscopically. They were separated into groups of icterus types: 24 (28.9%) dogs had pre-hepatic icterus, 45 (54.2%) had hepatic, 13 (15.7%) pre-hepatic and hepatic and one (1.2%) had post-hepatic icterus. Many factors were identified as a cause of icterus, including infectious agents (51/83), neoplasms (13/83), hepatic degeneration (11/83), chronic hepatic diseases (6/83), and obstructive causes (1/87). Among the infectious causes, leptospirosis, ehrlichiosis and disorders suggestive of septicemia were diagnosed. Neoplasms associated with icterus were cholangiocarcinoma, hemangiosarcoma and lymphoma. Other causes of icterus included degenerative diseases, such as lipidosis and glycogen degeneration. Hepatic fibrosis (cirrhosis) as a chronic disease and cholelithiasis also produced icterus. PCR was performed to confirm leptospirosis and ehrlichiosis. Samples of total DNA were used to amplify a fragment of a gene from Leptospira interrogans and Ehrlichia canis. In some dogs, co-infection of these agents was detected. The classification and identification of icterus etiologies in dogs is very important due to the number of diseases with this alteration, where ante mortem diagnosis is not always easily performed when some of these conditions are present.

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“…Higher levels of lipid peroxidation and decreased activities of superoxide dismutase, glutathione, and catalase have been shown in natural co-infections with CME, babesiosis, anaplamosis, and hepatozoonosis (36). Bottari et al (2) found higher levels of advanced oxidation protein products and the ferric-reducing ability of plasma in acute and subclinical phases of CME. The present study aims to represent the oxidative status with total and individual parameters and to show their relationship to the lipid profile.…”

Section: Co-infected (N = 23)mentioning

confidence: 98%

“…Oxidative stress and increased lipid peroxidation has been shown in dogs with CME because of free-radical activity and an impaired antioxidant system (2,8,36). Paraoxonase (PON-1) and arylesterase (ARE) are esterase enzymes with antioxidant properties in a complementary structure.…”

Section: Praca Oryginalnamentioning

confidence: 99%

Oxidative status and lipid profile in mono- and co-infection with canine monocytic ehrlichiosis

Gültekin

Ural

Paşa

et al. 2017

Medycyna Weterynaryjna

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SummaryVector-borne diseases in dogs are a major health problem of zoonotic importance. Canine monocytic ehrlichiosis (CME) is one of the most common vector-borne infections in dogs. Co-infections with vectorborne diseases are reported to cause more severe pathological effects than mono-infections. The current study investigates the effects of mono-and co-infections with CME on oxidative status and lipid profiles in dogs. Dogs with vector-borne diseases were divided into two groups, mono-infected (n = 22) and co-infected (n = 23) with CME, and compared to a healthy group (n = 20). Double and triple co-infections with other vector-borne diseases included A. phagocytophilum, L. infantum, and D. immitis. The serum lipid profile (high-density lipoprotein (HDL), low-density lipoprotein (LDL), cholesterol, and triglycerides) and the total and individual markers of oxidative status (total antioxidant capacity (TAC), total oxidant status (TOS), malondialdehyde (MDA), paraoxonase (PON-1), and arylesterase (ARE)) were evaluated. Significant decreases in PON-1 and ARE enzyme activities and HDL concentration, as well as increases in TOS level, MDA, LDL, and triglyceride concentrations were determined in both mono-and co-infected groups compared to the healthy control group. No significant differences between the groups were found in the TAC level or cholesterol concentration. In conclusion, alterations in lipid profile and increase in oxidative stress were observed in dogs with CME compared to the healthy control group, however, no significant differences were detected between mono-and co-infected dogs.

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Iron metabolism and oxidative profile of dogs naturally infected by Ehrlichia canis : Acute and subclinical disease

Cited by 11 publications

References 31 publications

Changes in Serum Biomarkers of Oxidative Stress in Cattle Vaccinated with Tick Recombinant Antigens: A Pilot Study

Changes in Serum Biomarkers of Oxidative Stress in Cattle Vaccinated with Tick Recombinant Antigens: A Pilot Study

Differential diagnoses in 83 dogs with icterus

Oxidative status and lipid profile in mono- and co-infection with canine monocytic ehrlichiosis

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