This study aimed to evaluate the effects of annatto seeds, linseed oil and their combination on DMI, apparent total tract digestibility, antioxidant capacity and milk composition of dairy cows. Four lactating Holstein cows (120 ± 43 days in milk; 15.98 ± 2.02 kg of milk/day, mean ± SD) were allocated in a 4 × 4 Latin square with a 2 × 2 factorial arrangement (with or without annatto seeds at 15 g/kg of dry matter (DM); with or without linseed oil at 30 g/kg of DM) and provided four different diets: control (no annatto seeds or linseed oil); annatto seeds (15 g/kg of DM); linseed oil (30 g/kg of DM); and a combination of both annatto seeds and linseed oil. Annatto seeds reduced DM intake, and milk yield, protein and lactose, but increased content of fat, total solids and short chain fatty acid, with no effect on total antioxidant capacity of milk. Linseed oil supplementation decreased medium chain fatty acid proportion and n-6/n-3 ratio, conversely it increased long chain fatty acids and n-3 fatty acid content of milk, ether extract intake and total-tract digestibility. Thus, linseed oil supplementation in dairy cow diets improved the milk FA profile but decreased milk fat concentration, whereas annatto seeds did not influence antioxidant capacity and depressed feed intake and milk yield.
This study was carried out to evaluate the white mulberry tree (Morus alba) as a feedstuff for ruminants in natura, as hay and as silage. A completely randomized design with five treatments and three replicates was used, where the treatments were five types of mulberry forage: (1) in natura after cutting; (2) hay; (3) silage of mulberry in natura; (4) silage of wilted mulberry and (5) silage of mulberry with ground ear corn. The content of crude protein (CP) did not differ between the silages (average of 19.03% of dry matter -DM). The CP content of the silages was higher than in mulberry hay (15.26% DM) and similar to mulberry after cutting (17.00% DM). The neutral detergent fiber (NDF) content did not differ between the silages (34.84% DM on average), but the NDF of silage with ear corn was lower than the mulberry hay (33.77 vs. 39.36% DM). The acid detergent fiber (ADF) content did not differ between silages and mulberry hay (30.66% DM on average). The lowest value for ADF was observed in the mulberry after cutting (26.06% DM). The aerobic stability of the silages was not broken during the seven days of aerobic exposure. The pH of silage of mulberry in natura was similar to silage of wilted mulberry (4.80 on average), while the silage with ear corn showed a lower pH value (4.58) compared to the other silages. The buffering capacity (BC) did not differ between the silage with ear corn and silage of wilted mulberry (84.91 meq 100 g DM -1 on average), while a lower value was observed for silage of mulberry in natura (56.69 meq 100 g DM -1 ). The highest value for ammonia nitrogen (NH 3 -N) was demonstrated in the silage of mulberry in natura (13.08% total N), and the lowest values were observed in the silage with ear corn and silage of wilted mulberry (7.49 and 9.58% total N, respectively). Silage with 11% of ground ear corn is the best alternative use of white mulberry tree as forage for ruminant feeding, since this form presented low fiber content and a fermentative pattern suitable for the process of forage conservation.
The objective of this study was to characterize calvings with low and high difficulty based on the productive and reproductive performance of dairy cows. Calvings were grouped in no calving assistance, calving with low assistance, and calving with high assistance. The original data set comprised 1,902 calving records obtained from a large dairy farm in Southeast Brazil. Factor analysis was applied using the SAS® Studio 3.8 statistical program through the factor procedure, considering the Multivariate Analysis category. Milk fat (0.92–0.79) and total solids (0.91–0.80) were strongly correlated with Factor 1. Calving interval (0.87– 0.68) and the number of AI (artificial inseminations) per conception (0.87–0.71) showed high correlations with Factor 2. Milk yield (0.84–0.76) and accumulated milk yield (0.84–0.77) were strongly correlated with Factor 3. Based on the results, we conclude that the three calving scenarios were characterized by well-defined and independent factors. Cows which required a high assistance at calving showed a lower variance explained by the model for milk fat and total solids contents, calving interval, and the number of AIs per conception.
We aimed to investigate the effects of calcium-ammonium nitrate (CAN) fed to lactating dairy goats on dry matter (DM) intake, digestibility of nutrients, milk properties (composition, antioxidant capacity, fatty acid profile, and nitrate residues), and ruminal fermentation parameters. Twelve lactating Saanen goats averaging 98.5 ± 13.1 days in milk, 53.5 ± 3.3 kg of body weight, and 2.53 ± 0.34 kg of milk/day were randomly assigned in four 3 × 3 Latin squares to receive the following diets: a control group (without CAN) with 7.3 g/kg DM of urea (URE), 10 g/kg DM of CAN (CAN10), and 20 g/kg DM of CAN (CAN20). Each period lasted 21 days, with 14 days for diet adaptation and seven days for data and sample collection. The DM intake, digestibility of nutrients, yields of milk, 3.5% fat-corrected milk, and energy-corrected milk were not affected by treatments. Similarly, there were no treatment effects on the yields and concentrations of milk fat, true protein, and lactose, along with minor effects on milk fatty acid profile. Total antioxidant capacity in milk was unaffected by treatments; however, concentration of conjugated dienes increased, while thiobarbituric acid reactive substances in milk decreased linearly. Nitrate and nitrite residues in milk were elevated by treatments, while the total of volatile fatty acids and ammonia-N concentration in the rumen were unaffected. Collectively, feeding CAN (up to 20 g/kg of DM) to lactating dairy goats did not affect feed intake, nutrient digestibility, and milk composition; however, it may increase milk lipid oxidation, as evidenced by increased conjugated diene concentration.
Resumo O objetivo deste estudo foi caracterizar os partos com leve ou severa dificuldade e diferenciá-los com base no desempenho produtivo e reprodutivo de vacas leiteiras. Os partos foram agrupados em partos sem assistência, partos com baixa assistência e partos com elevada assistência. O banco de dados original continha 1902 registro de partos que foram obtidos de uma grande fazenda comercial localizada no Sudeste do Brasil. A análise fatorial foi aplicada através do programa estatístico SAS® Studio 3.8 por meio de procedimento fatorial, considerando a categoria de análise multivariada. Os teores de gordura do leite (0,92– 0,79) e de sólidos totais (0,91–0,80) foram altamente correlacionados com o fator 1. Intervalo entre partos (0,87–0,68) e número de IA (inseminações artificiais) por concepção (0,87–0,71) apresentaram alta correlação com o fator 2. Produção de leite (0,84–0,76) e produção acumulada de leite (0,84–0,77) foram altamente correlacionados com o fator 3. Baseados nos resultados, é possível concluir que as três situações de parto foram caracterizadas por fatores independentes e bem definidos. Vacas que necessitaram de alta assistência ao parto apresentaram menor variância explicada pelo modelo para teores de gordura e sólidos totais do leite, intervalo entre partos e número de IA por concepção.
A composição química do leite sofre variações durante a vida produtiva da vaca, podendo ser influenciada por diversos fatores. Dentro deste aspecto, é possível que fatores intrínsecos como o horário de ordenha, esteja ligado às variações encontradas nos componentes do leite. Sendo assim, o objetivo deste estudo foi avaliar a variabilidade dos teores de gordura, proteína, sólidos totais e da produção de leite em diferentes turnos de ordenha e dias de coleta de amostras. Foram monitoradas 158 vacas Holandesas, multíparas, com 113 ± 64 dias em leite (DEL), peso corporal médio de 746,8 ± 57,1 kg e escore de condição corporal (ECC) médio de 3,16 ± 0,40. A coleta de amostras de leite ocorreu durante três dias consecutivos, entre os meses de outubro, novembro e dezembro de 2020. Foi encontrado efeito (p<0,01) do horário de ordenha para três dos quatro parâmetros avaliados neste estudo. A produção de leite foi maior (p<0,01) na ordenha da manhã (6:00 h). Os teores de gordura e sólidos totais do leite foram maiores (p<0,01) na ordenha da tarde (14:00 h). Não houve diferença estatística (p = 0,13) para os teores de proteína do leite entre os diferentes turnos de ordenha. Os resultados encontrados neste estudo indicam que ao coletar uma amostra de leite representativa da produção em 24 horas, deve ser priorizada a coleta de uma amostra composta das duas ou três ordenhas diárias e, se isto não for possível, fatores de ajuste devem ser estimados e adotados.
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