Adding lysolecithin to feed has reportedly improved the performance of broiler chickens. Lysolecithin is generated by phospholipase catalyzed hydrolysis of lecithin. The enzymatic reaction converts various phospholipids into the corresponding lysophospholipids, with lysophosphatidylcholine (LPC) one of the primary products. Here we compared supplementation with a commercial lysolecithin (Lysoforte®) with comparable levels of highly purified LPC for effects on broilers. Despite no differences in weight gain during the starter period, we discovered a significant increase in average villus length with lysolecithin and an increase in villus width with purified LPC. High-throughput gene expression microarray analyses revealed many more genes were regulated in the epithelium of the jejunum by lysolecithin compared to purified LPC. The most up-regulated genes and pathways were for collagen, extracellular matrix, and integrins. Staining sections of the jejunum with Picrosirius Red confirmed the increased deposition of collagen fibrils in the villi of broilers fed lysolecithin, but not purified LPC. Thus, lysolecithin elicits gene expression in the intestinal epithelium, leading to enhanced collagen deposition and villus length. Purified LPC alone as a supplement does not mimic these responses. Feed supplementation with lysolecithin triggers changes in the intestinal epithelium with the potential to improve overall gut health and performance.
Campylobacter is a bacterium that colonizes the lower gastrointestinal tract of poultry and may influence the intestinal environment to promote its survival. The objective of this study was to characterize the effects of Campylobacter challenge on the mRNA abundance of nutrient transporters and host defense peptides (HDP), such as the avian β-defensins (AvBD) and liver expressed antimicrobial peptide 2 (LEAP2). On the day of hatch, broiler chicks were challenged with one of three (106, 107, 108 colony-forming units, cfu) levels of Campylobacter jejuni. Quantitative PCR analysis revealed that there were dose-, tissue-, and age-specific changes in gene expression for both nutrient transporters and HDP. Expression of zinc transporter 1 (ZnT1) mRNA increased on d 7 in the duodenum, ileum, and cecum of birds challenged with 106 cfu of C. jejuni. At d 14, there was upregulation of the amino acid transporter bo,+AT mRNA in the duodenum, jejunum, and ileum of birds challenged with 106 cfu of C. jejuni. Other transporters such as EAAT3, GLUT2, SGLT1, and ZnT1 showed upregulation of mRNA in the ileum of the 106 cfu challenged group. There was a delayed response of the HDP to the C. jejuni challenge, with only a few HDP changed at d 7 but all HDP changed at d 14. At d 7, there was upregulation of AvBD10 mRNA in the duodenum of the 106 cfu challenged group but downregulation of AvBD10 in the ileum and AvBD12 and LEAP2 in the cecum of the 108 cfu challenged group. At d 14, there was upregulation of AvBD1, AvBD6, AvBD8, AvBD10, AvBD11, AvBD12, and AvBD13 mRNA in the ileum and cecum of the 106 cfu challenged group but not the 107 and 108 cfu challenged groups compared to control. These results indicated that at a low dose (106 cfu) of C. jejuni, intestinal cells increased nutrient transporter and AvBD mRNA abundance to try to counter the infection, but that at higher doses the cellular response was suppressed.
Simple SummaryChicken coops are rarely washed and can soil poultry carcasses with fecal bacteria that may make people sick. Our laboratory applied two commercially available products to experimentally contaminated coops. One product contained bleach, potassium hydroxide and a foaming agent. The other product contained vinegar and hydrogen peroxide and was mixed with a detergent. Both products were applied using a firefighting apparatus known as a compressed air foam system (CAFS). These materials were washed away using a garden hose or pressure washer as the treatments called for. Surface swabs were collected prior to and after each treatment to determine the reduction of bacteria on the surface, which would be an indicator of sanitation. We found that both treatments significantly made the surface cleaner when compared to water alone. The application of these products via a CAFS may be a practical and expedient way to clean and disinfect poultry cages.AbstractTransport coops are infrequently washed and have been demonstrated to cross-contaminate broiler carcasses. We hypothesized that peracetic acid or a chlorinated cleaner, commonly used within poultry processing plants, can also be used to disinfect transport coops when applied via a compressed air foam system (CAFS). A mixture of fresh layer manure and concentrated Salmonella Typhimurium (ST) was evenly applied to the floors of four pre-cleaned transport coops and allowed to dry for thirty minutes. Treatments consisted of a (1) water rinse only, (2) product application with a water rinse, (3) product application followed by power washing and (4) power washing followed by application of product. Each foaming treatment was applied with a compressed air foam system and allowed 10 min of contact time. Samples were aseptically collected from the transport coops prior to and following treatment using a sterile 2 × 2-inch stainless steel template and a gauze swab pre-enriched with buffered peptone water. The chlorinated cleaner significantly (p < 0.05) reduced aerobic bacteria and ST by 3.18 to 4.84 logs across application methods. The peroxyacetic acid (PAA) disinfectant significantly (p < 0.05) reduced aerobic bacteria and ST by 3.99 to 5.17 logs across application methods. These data indicate that a compressed air foam system may be used in combination with a commercially available cleaner or disinfectant to reduce aerobic bacteria and ST on the surfaces of commercial poultry transport coops.
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