Research Note: Internal organ colonization by Salmonella Enteritidis in experimentally infected layer pullets reared at different stocking densities in indoor cage-free housing

Gast, Richard K.; Jones, D. R.; Guraya, Rupa; Garcia, Javier S.; Karcher, Darrin M.

doi:10.1016/j.psj.2022.102104

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…However, they can sometimes cause fatal diseases through viral and bacterial infections. Salmonella, transmitted through contact with chicken feces, harms human public health and food safety, and avian influenza reduces chicken populations [10,19,20]. We aimed to develop a clean alternative protein supply method using SPF embryos from laying hens.…”

Section: Discussionmentioning

confidence: 99%

“…Among livestock, chickens have been used as in vitro or in vivo experimental models in various fields, including basic sciences, the food industry, and pharmaceutical fields for human beings [9]. Despite its importance, Salmonella, a pathogen in the digestive tract or feces of chickens, often causes food-borne human diseases and adversely affects the economy due to reduced chicken productivity [10]. Therefore, in vitro cell culture engineering studies using embryos or chickens produced under specific-pathogen-free (SPF) conditions are required to overcome these limitations.…”

Section: Introductionmentioning

confidence: 99%

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Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture

Seo

Lee

et al. 2023

Animals

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This study examined the potential benefits of male specific-pathogen-free (SPF) White Leghorn embryos in cellular agriculture for sustainable and ethical poultry meat production—addressing traditional farming challenges, including disease outbreaks of Salmonella and Avian influenza. We isolated myogenic precursor cells (MPCs) from the thigh muscles (Musculus femoris) of 12.5-day-old embryos from 10 SPF White Leghorns that tested negative for Salmonella. We randomly selected MPCs from three males and three females, isolated them using a modified pre-plating (pp) method, and compared their in vitro development. After 1 h (pp1) and 2 h (pp2) of incubation, they were transferred to a new dish to remove fast-adhering cells and cultured (pp3). Isolated MPCs had a 69% positive reaction to Pax7. During proliferation, no differences were observed in PAX7, MYF5, or MYOD expression between the male and female MPCs. However, after five days of differentiation, the expression of late myogenic factors—MYOG and MYF6—significantly increased in all MPCs. Notably, MYOG expression was 1.9 times higher in female than in male MPCs. This impacted MYMK’s expression pattern. Despite this, the myotube fusion index did not differ between the sexes. Muscle cells from male SPF-laying chicken embryos are promising for developing clean animal-cell-derived protein sources via resource recycling.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture

Seo

Lee

et al. 2023

Animals

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“…Contrary to intensive farming systems, contaminations of feeds and water are high, which later affect the safety of food products along the value chain. The infected hens raised in cages are more exposed to contaminated environments and more likely to lay infected eggs and, subsequently, chicks which acquire Salmonella through the hen's reproductive system [ 185 ]. In an aquatic environment, contaminated seafood such as fish, shrimp, clams, mussels, oysters, crabs, lobsters, squid, cuttlefish, and octopus often are from contaminated water and surroundings where seafood is handled [ 186 ].…”

Section: Prevention Of Salmonella Infectionsmentioning

confidence: 99%

Prevention and Control of Human Salmonella enterica Infections: An Implication in Food Safety

Mkangara

2023

International Journal of Food Science

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Salmonella is a foodborne zoonotic pathogen causing diarrhoeal disease to humans after consuming contaminated water, animal, and plant products. The bacterium is the third leading cause of human death among diarrhoeal diseases worldwide. Therefore, human salmonellosis is of public health concern demanding integrated interventions against the causative agent, Salmonella enterica. The prevention of salmonellosis in humans is intricate due to several factors, including an immune-stable individual infected with S. enterica continuing to shed live bacteria without showing any clinical signs. Similarly, the asymptomatic Salmonella animals are the source of salmonellosis in humans after consuming contaminated food products. Furthermore, the contaminated products of plant and animal origin are a menace in food industries due to Salmonella biofilms, which enhance colonization, persistence, and survival of bacteria on equipment. The contaminated food products resulting from bacteria on equipment offset the economic competition of food industries and partner institutions in international business. The most worldwide prevalent broad-range Salmonella serovars affecting humans are Salmonella Typhimurium and Salmonella Enteritidis, and poultry products, among others, are the primary source of infection. The broader range of Salmonella serovars creates concern over multiple strategies for preventing and controlling Salmonella contamination in foods to enhance food safety for humans. Among the strategies for preventing and controlling Salmonella spread in animal and plant products include biosecurity measures, isolation and quarantine, epidemiological surveillance, farming systems, herbs and spices, and vaccination. Other measures are the application of phages, probiotics, prebiotics, and nanoparticles reduced and capped with antimicrobial agents. Therefore, Salmonella-free products, such as beef, pork, poultry meat, eggs, milk, and plant foods, such as vegetables and fruits, will prevent humans from Salmonella infection. This review explains Salmonella infection in humans caused by consuming contaminated foods and the interventions against Salmonella contamination in foods to enhance food safety and quality for humans.

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“…S. Enteritidis is also considered a pathogen of poultry . Although it generally causes no symptom or mild illness in chickens, S. Enteritidis colonizes gastrointestinal and reproductive tracts leading to contaminated chicken meats and eggs which are the main sources of foodborne salmonellosis in humans (Campioni et al, 2014;Gast et al, 2022). An increasing trend of S. Enteritidis infection in humans has been observed globally including Thailand (Whistler et al, 2018;Balasubramanian et al, 2019) and S. Enteritidis in poultry farms has been closely and legally monitored (DLD, 2010).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of ciprofloxacin resistance, plasmid-mediated quinolone resistance genes and virulence factors in Salmonella enterica serovar Enteritidis isolated from broilers in Thailand isolated from broilers in Thailand

Suwannachot

Ketphan

Jittimanee

et al. 2023

VIS

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Salmonella Enteritidis is one of the most common serovars associated with gastroenteritis in humans. Fluoroquinolone resistance in non-typhoidal Salmonella including S. Enteritidis has increased globally and is considered as a threat to public health. In this study, we aimed to investigate the occurrence of ciprofloxacin resistance and plasmid-mediated quinolone resistance (PMQR) genes, and examine virulence gene profiles of 69 S. Enteritidis isolates recovered from 46 boot swab and 23 intestinal samples collected from 69 commercial intensive broiler farms in Thailand. Ciprofloxacin susceptibility of these isolates was determined using microbroth dilution method. PCR was used to detect 5 common PMQR genes (qnrA, qnrB, qnrS, aac(6')-Ib-cr and qepA) and 12 important virulence genes (agfA, invA, spaN, prgH, sitC, ssaQ, mgtC, sopB, sifA, tolC, cdtB and spvC). All S. Enteritidis showed reduced susceptibility to ciprofloxacin, with the MIC values of 0.125-0.50 µg/mL. However, these isolates did not carry PMQR genes investigated. The same virulence profile was observed among 69 S. Enteritidis isolates in which 11 virulence genes, except cdtB, were detected. The presence of virulence genes identified in invasive salmonellosis in the S. Enteritidis isolates with reduced susceptibility to ciprofloxacin could be of public health concerns. Our findings underline the need for constant monitoring of ciprofloxacin-resistant S. Enteritidis in poultry production chain to reduce public health risk.

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Research Note: Internal organ colonization by Salmonella Enteritidis in experimentally infected layer pullets reared at different stocking densities in indoor cage-free housing

Cited by 6 publications

References 10 publications

Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture

Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture

Prevention and Control of Human Salmonella enterica Infections: An Implication in Food Safety

Occurrence of ciprofloxacin resistance, plasmid-mediated quinolone resistance genes and virulence factors in Salmonella enterica serovar Enteritidis isolated from broilers in Thailand isolated from broilers in Thailand

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