An in vitro experiment was carried out to evaluate the phytoremediation potentials of two somatic embryo-derived ecotypes of Arundo donax-BL (American ecotype) and 20SZ (Hungarian ecotype)-of copper from synthetic wastewater. The two ecotypes were grown under sterile conditions in tubes containing a nutrient solution supplied with increasing doses of Cu (0, 1, 2, 3, 5, 10, and 26.8 mg L(-1)) for 6 weeks. The translocation and bioaccumulation factors and removal rate were estimated. In general, increasing Cu concentration in nutrient solution slightly decreased root, stem and leaf biomass without toxicity symptoms up to 26.8 mg L(-1). Moreover, both ecotypes showed high Cu removal efficiency from aqueous solution. However, Cu removal rate ranged between 96.6 to 98.8% for BL ecotype and 97 to 100% for 20SZ ecotype. Data illustrated that both BL and 20SZ ecotypes may be employed to treat Cu-contaminated water bodies up to 26.8 mg L(-1).
The production/consumption of greenhouse gases (GHG) in soils are of great importance in global warming, but the involved soil physico-chemical and biological characteristics affecting GHG production and consumption potentials are poorly understood in different land-use types. Carbon dioxide (CO 2 ), nitrous oxide (N 2 O) and methane (CH 4 ) production/consumption potentials from four land-use types and 10 soil types in central Japan and eastern Hungary, and their relationships with soil characteristics, were investigated. The average of CO 2 production in Japanese soils was significantly higher than that of Hungarian soils due to the relatively higher microbial biomass carbon (MBC) content. N 2 O production from both countries' soils did not exhibit a significant difference. Most soils except Japanese paddy and soybean soils showed the potentials for CH 4 consumption. Forest and grassland soils had relatively higher CO 2 and N 2 O production than orchard and cropland soils for both countries. From regression analyses, it could be concluded that soil total nitrogen (TN) and ammonium-nitrogen (NH 4 + -N) account for 40.8% and 25.5% variations of the soils' CO 2 and N 2 O productions, respectively. The CH 4 consumption was positively correlated with soil carbon/nitrogen (C/N) ratio, while soil MBC availability could account for 15% variation of CH 4 consumption under aerobic conditions.
Cultivating the top 0-25 cm soil layer by ploughing cultivating method requires considerable energy, labor, and additional costs. Also, the larger soil surface caused by cultivation, the moisture content of soil can be lost easier. Therefore, in recent years soil loosening cultivation has become gained popularity, particularly to protect the moisture content of soil and reduce the risk of desertification. At the Experimental Station of Debrecen University, known as Látókép (a name, which corresponds approximately to visual image), two cultivation methods have been applied for research: (i) conventional ploughing and (ii) strip and streaked loosening cultivation methods (and variation of this method applying satellite determination of position, RTK system). In this paper, total number of bacteria, soil respiration, biomass carbon and nitrogen, net nitrification, and dehydrogenase activity were measured under irrigated and non-irrigated conditions. The aim of the research was to evaluate the effects of the various cultivation methods. Soil samples were collected in spring (May) and autumn (September). Microbiological effects on the soil resulted from various cultivation methods were compared. Results demonstrate that the loosening cultivation method (strip tillage with loosening) exerts a more favorable effect on the parameters of soil biological activity than the conventional ploughing system. The most significant effect of loosening cultivation system was experienced in the increase of microbial biomass carbon (MBC) and nitrogen (MBN) by over 80%, along with an intensified dehydrogenase activity. Loosening system yielded positive effects on the other examined biological parameters, except for the total bacteria number and soil respiration.
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