The epidemiology, clinical signs, and pathology of 42 outbreaks of enzootic calcinosis (EC) in cattle, sheep and goats in Mato Grosso (19), Distrito Federal (19), Goiás, (6), Minas Gerais (2) and Tocantins (1), Brazil, [date not given] are presented. Additionally, Ageratum conyzoides, Chamaecrista desvauxii [Cassia desvauxii], Cissus erosa, Elephantopus mollis, Eragrostis sp., Eupatorium odoratum [Chromolaena odorata], Hymenaea courbaril, Jatropha sp., Ludwigia octovalvis, Mimosa hirsutissima, Murdania nudiflora, Rhynchanthera novemnervia, Scoparia dulcis, Sida santaremensis, Tibouchina sp., Vernonia brasiliana, Waltheria sp., Arachis sp., Cnidoscolus cnicadendron, Eleusine tristachya, Ludwigia leptocarpa, Ludwigia sp., Mimosa pellita, Sida cerradoensis, Sida ciliaris, Sida spinosa and Vernonia ferruginea as the possible causes for the outbreaks were experimentally administered to rabbits and sheep. It was shown that none of the 33 rabbits and 14 sheep that ingested the selected weeds to detect calcinogenic effect of the plants developed clinical changes, and mineralization of soft tissues was not observed during necropsies or histologic examination. Serum Ca and P concentrations were within normal values.