Relatam-se os achados epidemiológicos, clínicos, patológicos e micológicos de conidiobolomicose em ovinos no Estado de Mato Grosso. A doença ocorreu em uma propriedade no município de Nobres, em um rebanho com 40 ovelhas adultas, entre os meses de janeiro a junho de 2007. Aproximadamente 30% dos ovinos da propriedade adoeceram e todos os doentes morreram em curso clínico de 2-5 semanas. O quadro clínico foi caracterizado por apatia, emagrecimento, dificuldade respiratória com dispnéia, respiração ruidosa e oral, secreção nasal mucosa ou sero-sanguinolenta, exoftalmia unilateral, por vezes com cegueira, e morte. Havia desaparecimento de etmoturbinados com substituição por tecido bran-cacento, finamente granular, multilobulado e friável infil-trando-se na lâmina cribiforme, no septo nasal e nas coa-nas em todos os ovinos necropsiados. Lesões similares foram encontradas em linfonodos regionais (2 casos), pulmões (3), encéfalo (2) e em linfonodos do abomaso (1). Microscopicamente havia inflamação granulomatosa da região rinocerebral, caracterizada por necrose, proliferação de tecido conjuntivo, infiltrado de neutrófilos, eosinófilos, células epitelióides e células gigantes multinucleadas freqüentemente circundando material Splendori-Hoeppli, onde havia imagens negativas de hifas. Na impregnação pela Prata-Metenamina, as hifas tinham septos e ramificações escassas e irregulares, com dilatação balonosa terminal e com forte demarcação de contornos. Iso-lou-se de tecido nasal de quatro ovinos Conidiobolus sp.
The rhinitis and granulomatous lymphadenitis was characterised histopathologically by caseous necrosis with proliferation of connective tissue, a neutrophilic and eosinophilic infiltrate, epithelioid cells, multinucleate giant cells, and irregular, moderately septate and ramified hyphae, frequently surrounded by abundant granular and eosinophilic material with radial disposition (Splendore-Hoeppli phenomenon). Lesions from the dorsal and lateral regions of the nose were collected aseptically and transported to the laboratory at room temperature. The samples were placed on to 2 per cent Sabouraud's dextrose agar and incubated at 37°C. Forty-eight hours later, the cultures demonstrated a slight growth of white colonies with submerged root-like elements. Later, it was possible to observe organisms morphologically compatible with P insidiosum following the induction of zoosporo genesis, performed as described by Santurio and others (2003). The identity of the pathogen was confirmed in all four cases through the amplification of DNA by PCR, as described by Rodrigues and others (2006). The extensive ulcerative lesions observed in the sheep resembled the cutaneous lesions described in sheep by Tabosa and others (2004), in horses by Alfaro and Mendoza (1990), in dogs by Thomas and Lewis (1998) and in cattle by Santurio and others (1998). The histopathological findings of granulomatous eosinophilic lesions with many giant cells and Splendore-Hoeppli material involving P insidiosum hyphae are consistent with those observed in previous studies involving sheep from northeastern Brazil (Tabosa and
Outbreaks of tetanus, in which 297 beef cattle and 50 sheep were affected and died, were associated with the injection of a Clostridium tetani-contaminated anthelmintic (disophenol). The disease was observed on five farms in Rio Grande Sul, Brazil.
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