The objectives of the study were to develop a reverse-phase high-performance liquid chromatography method for the simultaneous analyses of trans-/cis-resveratrol, emodin, and quercetin and to determine the concentrations of these polyphenols in 28 resveratrol supplements. Samples were separated within 15 min in a C18 reversed-phase column using mobile phases containing 0.1% formic acid and methanol/0.1% formic acid. The calibration graphs for all four compounds were linear from 0.1 to 410 μg/mL (r2=0.99). The concentration of resveratrol as stated on the labels was often different from the analytical results, with 21 and 11% of the total supplements having low or high values, respectively. Many of the supplements also contained variable but generally low levels of emodin, a compound known to cause diarrhea. The proposed method is a rapid, sensitive, accurate, and cost-effective procedure that can be used for the simultaneous quantification of four polyphenols in resveratrol supplements.
The objective was to examine effects of whole and fractions of pinto beans and peas on diet induced dyslipidemia. Hamsters were randomly assigned to consume high fat diet (n=12 each) including control (CON); bean starch (BS); bean fibre (BF); whole bean (WB); cooked whole bean (CWB); inner pea fibre (IPF); pea hull fibre (PHF); pea protein isolate (PPI); pea protein concentrate (PPC); or whole pea (WP) for 4 wk. Reduced feed intake (FI) and body weight gain (BWG) were observed with WB (FI: p=0.030; BWG: p<0.001), BS (FI: p=0.041; BWG: p<0.001) and BF (FI: p=0.037; BWG: p<0.001) compared to CON. However, both FI and BWG did not differ between CWB and CON. Plasma total, HDL and non‐HDL cholesterol, triglyceride and glucose concentrations were lower (p<0.05) in CWB, WB, BS and BF compared to CON. Furthermore, no changes in plasma lipids were observed with BF and BS compared to WB. No changes in FI, BWG and plasma glucose and lipid profile were observed with any of the peas supplemented groups compared to CON. A positive hemagglutination was observed with raw WB, BS and BF, but not CWB, against rabbit erythrocytes. Data suggest that, while whole and fractionated peas failed to alter plasma lipids and glucose levels, fractions and whole beans possess lipid lowering and hypoglycemic effects in hamsters, but must be cooked prior to intake. Results indicate that bean consumption can improve blood lipids and reduce cardiovascular disease risk
Grant Funding Source: Supported by Pulse Canada; Agriculture and Agri‐Food Canada
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