EVLA for varicose veins is safe and effective compared with HLS in a two-year range. More randomized controlled studies follow-up results are needed to clarify long-term recurrence (5 years).

Yes-associated protein (YAP) has been implicated as an oncogene in multiple human cancers. In the present study, human gastric adenocarcinoma tissues of different grades (N=78) were collected and the mRNA and protein expression of YAP and phosphorylated YAP (p-YAP) in gastric adenocarcinomas were evaluated using immunohistochemistry, Real-time PCR and Western blot assays. Then, human gastric cancer SGC-7901 cells were stably transfected with lentivirus-mediated YAP small hairpin RNA (shRNA). The expression levels ofYAP,proliferating cell nuclear antigen (PCNA) and metalloproteinase-2 (MMP-2) were detected and the effects of shRNA-mediated knockdown ofYAP on cell proliferation and metastasis were assessed in gastric cancer cells. As a result, the expression ofYAP was observed in 69.23% gastric adenocarcinoma tissues, elevating with the ascending order of tumor malignancy. Knockdown of YAP could down-regulated the expression of PCNA and MMP-2, and inhibit the proliferation and metastasis of gastric cancer cells. In conclusion, YAP is strongly expressed in gastric adenocarcinomas, and knockdown of YAP may inhibit gastric cancer cell proliferation and metastasis through downregulation of PCNA and MMP-2 expression, suggesting that YAP represents an important therapeutic target in human gastric cancer.

The effect of an external transverse magnetic field on ionization injection of electrons in a laser wakefield accelerator (LWFA) is investigated by theoretical analysis and particle-in-cell simulations. On application of a few tens of Tesla magnetic field, both the electron trapping condition and the wakefield structure changes significantly such that injection occurs over a shorter distance and at an enhanced rate. Furthermore, beam loading is compensated for, as a result of the intrinsic trapezoidal-shaped longitudinal charge density profile of injected electrons. The nonlinear ionization injection and consequent compensation of beam loading lead to a reduction in the energy spread and an enhancement of both the charge and final peak energy of the electron beam from a LWFA immersed in the magnetic field.

Early detection and diagnosis of colorectal cancer (CRC) are closely related to a better therapeutic outcome, and the five-year survival rate ofearly CRC is over 90%. Though endoscopic minimally invasive treatment has become a quick and effective therapy for early CRC, endoscopic biopsies are usually not deep enough to obtain tissues from the submucosal layer and it is difficult to determine whether early CRC has infiltrated into the submucosa. Therefore, in the present study, we constructed tumor models of early submucosal non-invasive CRC (SNICRC) and submucosal invasive CRC (SICRC) in Fischer-344 rats induced by N-methyl-N-nitrosourea (MNU). The differentially-expressed proteins were analyzed and identified in SNICRC, SICRC and normal control (NC) tissues using highly sensitive twodimensional differential gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS). Proteomic data revealed 132 protein spots between SNICRC and SICRC, 162 protein spots between SICRC and NC and 154 protein spots between SNICRC and NC which were found differentially expressed. These differential spots were picked, in-gel digested and peptide mass fingerprints were obtained by MALDI-TOF-MS/MS. Finally, five differentially-expressed proteins in SNICRC, SICRC and NC were identified, and increases in Transgelin, peptidylprolyl isomerase A (PPIA) and tropomyosin alpha isoform d were observed, while decreases in carbonic anhydrase 2 (CAlI) and an unnamed protein were detected in SICRC compared with SNICRC and NC. Furthermore, Fluorescence-based quantitative polymerase chain reaction (FQ-PCR), Western blotting and immunohistochemistry assays also revealed significant up regulation of Transgelin expression and down-regulation of CAlI expression in SICRC tissues. In conclusion,~D-DIGE is confirmed to be an efficient strategy that enables us to identify differentiallyexpressed proteins between early SNICRC and SICRC. The potential biomarkers such as Transgelin and CAlI may be used for the detection of early SICRC Colorectal cancer (CRC) is a major cause of cancer incidence and mortalit y, and there are nearly one million new cases of CRC diagnosed worldwide each year and half a million deaths (1). The past two decades have brought major advances in CRC screening and therapeuti cs including advances in colonoscop y therapy. However, it is hard for endoscopic examination or other means to detect early CRC lesions.Thus, research focuses on specific strategies

High mobility group box 1 (HMGB1) has been proved to be implicated in a variety of cell physiological and pathological behaviors including immune response, inflammation and cancer. Accumulating evidence suggests that HMGB1 plays a critical role in the development and progression of multiple malignancies. However, the clinical significance and prognosis of HMGB1 expression in some cancers remain controversial. The present study aimed to investigate whether overexpression of HMGB1 is an independent prognostic factor in patients with gastric cancer. The correlation of HMGB1 expression with clinicopathologic characteristics and prognosis was assessed by immunohistochemical assay through tissue microarray procedure in 50 primary gastric cancer cases. Our results indicated that the positive expression of HMGB1 was significantly increased in the nucleus of gastric cancer tissues compared with the adjacent non-cancerous tissues (ANCT) (64.0% vs 44.0%, P=0.025), but was not linked to the clinicopathologic features, including the TNM stage (P=0.533) and metastatic lymph node (P=0.771), in patients with gastric cancer. Kapalan-Meier and log-rank analysis demonstrated that overexpression of HMGB1 did not exert significant impact on the overall survival of patients with gastric cancer (P=0.805). Furthermore, Cox regression analysis showed that high HMGB1 protein expression did not represent an independent risk factor for patients with gastric cancer (P=0.677). Taken together, our findings suggest that high expression of HMGB1 is not correlated with the clinicopathologic characteristics of gastric cancer, and can not serve as an independent prognostic biomarker for patients with gastric cancer.

A directional hot electron jet with energy higher than 100 keV was generated along the laser propagation direction from Ar clusters irradiated with a laser pulse of duration 28 fs and intensity 1 x 10(17) W/cm(2). The hot electron jet was detected only with linearly polarized laser pulses, not with circularly polarized pulses. Channel betatron resonance is believed to be the main accelerating mechanism for this directional hot electron jet.

Corpuscles of stannius (CS), a small endocrine gland located on the ventral surface of the kidneys of bony fishes, is extracted to purify stanniocalcins (STCs), which inhibit whole-body Ca2+ influx in the whole body, especially in gill and gut. CSs were first considered to be unique endocrine glands in fish, but were further verified to appear in mammalians, indicating that STCs may function in mammalians as calcium regulation, oxidative stress, anti-inflammation, angiogenesis, ischemia reperfusion, etc. Moreover, the relationship and molecular mechanisms of STC1 expression in cancer become the studied focus and front field. STC2 as a STC1 homolog is identified by searching for related sequences in expressed sequence tag databases. Although mammalian STC1 and STC2 are not expressed ubiquitously in tissue cells, they are distributed in a wide variety of tissues and organs including cancer, and play an important role in tumor development and progression. Molecular structureSTC is present in all vertebrates as two isoforms, STC1 and STC2, encoded by separate genes. 1 STC1, originally described as an antihypercalcemic hormone in fish, is highly expressed in differentiated mammalian neurons. STC1 adopts a dimeric and slightly elongated structure with a high content of alpha-helices. 2 STC2, a homolog of STC1, is a 56kD glycoprotein hormone that confers calcitonin-like activity. A widespread distribution of SCT1 and STC2 is present in mammals and fish, in which the highest amount of STC mRNA is in the heart but lower amounts are found in the neural complex, branchial basket, and endostyle. STCs have a conserved pattern of 10 cysteines in Evolution and functions of stanniocalcins in cancer S-J Chu, 1 J Zhang, 2 R Zhang, 2 W-W Lu 2 and J-S Zhu 2 Abstract Stanniocalcin (STC), first isolated from the corpuscles of stannius of teleost fishes, was originally known for its regulation on calcium/phosphate transport. Increasing evidence demonstrates that STCs display the important function in some physiological and pathological behaviors such as calcium regulation, oxidative stress, anti-inflammation, angiogenesis, ischemia reperfusion, nerve diseases, etc. Moreover, STCs are implicated in the development and progression of multiple malignancies through promoting cell growth, proliferation, invasion, metastasis, and apoptotic escape. Some studies have shown that NF-κB upregulates STC expression, thereby activating the downstream HIF-1/ERK1/2 signaling pathway, enhancing the transcriptional activity of tumor-related factors (MMP-2/9, cyclinD1, Bcl-2, N-cadherin, etc) and contributing to tumorigenesis. Here, this brief review describes recent progress of STCs in mammalians, focused mainly on their critical functions in cancer.

Oxymatrine is a kind of alkaloid extracted from traditional Chinese herb Sophora flavescens Ait. It has been proved to exert various biological activities such as anti-angiogenesis, proliferation-inhibiting, apoptosis-promoting, analgesicstrengthening, and anti-metastasis. The biological activities are related with inhibition of angiogenesis-associated factors, regulation of related signaling pathway and protein expression, synergistic effects with chemotherapy drug, cell cycle arrest and inhibition of voltage-activated K+ channel. In this review, we summarize the recent investigations of oxymatrine in cancer therapy so as to provide references for further study and clinical therapy.