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The work herein described was undertaken in connection with a study of the effect of fast-freezing upon the bacterial flora of mackerel. The purpose of the experiments was to ascertain the effects of the low temperatures used in the process upon-the characteristics of growth of these bacteria which represent a major portion of the bacterial flora of the fish. RELATIONSHIP BETWEEN GROWTH OF BACTERIAL FLORA OF MACKEXEL AND A RANGE OF TEMPERATURE' This work was aided by an Ellen Browning Scripps Fellowship. a Present address : Lederle Laboratorie~, Pearl River, N. Y. All temperatures refer to-Centigrade scale. 257 FOOD RESEARCH; VOL. 9, No. 4.