Twenty isolates of Phytophthora infestans from potato and twenty-two from tomato, collected in Uganda and Kenya in 1995, were compared for dilocus allozyme genotype, mitochondrial DNA (mtDNA) haplotype, mating type and restriction fragment length polymorphism (RFLP) fingerprint using probe RG57. Based on RFLP fingerprint and mtDNA haplotype, all isolates were classified in the US21 clonal lineage. Nonetheless, isolates from potato differed from isolates from tomato in several characteristics. Isolates from potato had the 86/100 glucose-6-phosphate isomerase (Gpi) genotype, while those from tomato were 100/100, which represents a variant of US21 that had been identified previously as US21.7. Furthermore, while pure cultures of the pathogen were acquired from infected potato leaflets by first growing the isolates on potato tuber slices, this approach failed with infected tomato tissue because the isolates grew poorly on this medium. Tomato isolates were eventually purified using a selective medium. Six isolates from each host were compared for the diameter of lesions they produced on three tomato and three potato cultivars in one or two detached-leaf assays (four isolates from the first test were repeated in the second). On potato leaflets, isolates from potato caused larger lesions than isolates from tomato. On tomato leaflets, isolates from that host caused larger lesions than did isolates from potato, but the difference was significant in only one test. The interaction between source of inoculum (potato or tomato) and inoculated host (potato or tomato) was significant in both tests. Isolates from tomato were highly biotrophic on tomato leaflets, producing little or no necrosis during the seven days following infection, even though abundant sporulation could be seen. In contrast, isolates from potato sporulated less abundantly on tomato leaflets and produced darkly pigmented lesions that were most visible on the adaxial side of the leaflets. Nonetheless, all isolates infected and sporulated on both hosts, indicating that host adaptation is not determined by an ability to cause disease but rather by quantitative differences in pathogenic fitness. Assessment of Gpi banding patterns, mtDNA haplotype and RFLP fingerprint of 39 isolates from potato collected in Uganda and Kenya in 1997 indicated that the population had not changed on this host. The population of P. infestans from Kenya and Uganda provides an interesting model for the study of quantitative host adaptation.
Cassava adaptive trial was planted in ten sub counties across West Nile Agro-ecological Zone (WNAEZ) with six cassava varieties to test their performance and reactions to major pests and diseases present in the Zone. Th six cassava varieties comprised of improved (TME 14 and 204, NASE 13 and 14 and Akena -TMS I92/00067) and a local (Abiria) were selected based on their availability and preference in the region. The experiment was planted in RCBD design with three replicates. The experiment was planted in ten locations with the plot size was 6x6 metres. Results showed Cassava green mite (CGM), Cassava mosaic disease (CMD) and cassava bacterial blight (CBB) as major pest and diseases across all sites. Cassava Anthracnose (CA) and Cassava mealy bug (CM) were not present in the study sites. Cassava brown streak disease (CBSD) foliar symptoms was observed in three locations of Dranya s/c, Gimara s/c and Nyaravuru s/c on the three varieties of TME 204, TMS-I92/00067 and TME 14, whereas CBSD root necrosis was seen across all sites on TME 204, TMS-I92/00067, NASE 13, TME 14 and the Local except on NASE 14. In terms of yields, results showed that it was highest in TMS-I92/00067 (53.0 t/ha), TME 204 (46.0 t/ha), NASE 14 (39.4 t/ha), TME 14 (34.6 t/ha), NASE 13 (33.4 t/ha) and the local (22.7 t/ha) in that order. Farmers' ranking of the studied cassava varieties in order of preference was in the order of NASE 14, TME 204, TMS-I92/00067, TME 14, NASE 13 and the Local. In conclusion, absence of both foliar and root symptoms on NASE 14 across all sites indicated that this variety is still tolerant to CBSD and can still be multiplied for production in West Nile Agro-ecological Zone.
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