The APS Journal Legacy Content is the corpus of 100 years of historical scientific research from the American Physiological Society research journals. This package goes back to the first issue of each of the APS journals including the American Journal of Physiology, first published in 1898. The full text scanned images of the printed pages are easily searchable. Downloads quickly in PDF format.
Effects of ethylenediaminetetraacetate (EDTA), citrate, ascorbate, succinate, and sorbitol on the absorption and postabsorptive distribution of Fe59Cl3 in the rat were studied, using an isolated loop technique. Citrate most effectively promoted iron absorption, with ascorbate, EDTA, and succinate following in that order. Sorbitol seemed without effect. Control animals with access to food until time of experiment absorbed more radioiron than did fasted controls. Partial trypsin hydrolysis permitted demonstration that succinate-exposed gut cells most avidly retained iron, while EDTA led to least cellular retention. Postabsorptive distribution among blood, liver, spleen, long bones, kidneys, and urine was unremarkable except that EDTA promoted marked radioiron urinary excretion. EDTA may pass through the gut wall as an intact iron chelate. A pattern of actions during iron absorption is proposed for EDTA, citrate, ascorbate, and succinate.
The effects in rats, of age, iron-deficiency anemia, and ascorbic acid, citrate, fluoride, and ethylenediaminetetraacetate (EDTA) on enteric radioiron transport were studied in vitro by an everted gut-sac technique. Sacs from young animals transported more than those from older ones. Proximal jejunal sacs from anemic animals transported more than similar sacs from nonanemic rats, but the reverse effect appeared in sacs formed from proximal duodenum. When added to media containing ascorbic acid or citrate, fluoride depressed transport as did anaerobic incubation in the presence of ascorbic acid. Anaerobic incubation in the presence of EDTA appeared to permit elevated transport. Ascorbic acid, citrate, and EDTA all enhanced the level of Fe59 appearing in serosal media. These results appear to agree with previously established in vivo phenomena and tend to validate the in vitro method as one of promise for further studies of factors affecting iron absorption and of the mechanism of iron absorption.
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