Field surveys were carried out in three Bhut Jolokia growing districts viz., Sivasagar, Jorhat, Golaghat of Upper Assam to assess the incidence of viral diseases during 2009-11. The incidence of virus diseases varied in farmer's field with an average ranged from 8-72% .Four different viruses were identified based on symptomatology, transmission, host range and serological assays. The mosaic disease was detected to be cucumovirus, potyvirus leaf and stem necrosis disease to be caused by tospovirus and leaf curl disease caused by begomovirus. Cucumovirus , Potyvirus and Tospovirus were easily transmitted by grafting and sap inoculation to Bhut Jolokia and other hosts tested. However, begomovirus was found to be graft transmissible whereas sap inoculation failed to produce any symptom on host plants. Mixed infection of Cucumovirus and Potyvirus was separated by sap inoculation on Datura stramonium. Cucumovirus produced local lesion while Potyvirus produced chlorotic lesion on D. stramonium. DAS-ELISA revealed highest incidence of Potyvirus (87.50%) followed by Cucumovirus (75.00%) and Tospovirus (62.50%) in Golaghat while lowest incidence (31.25-33.33%) of virus diseases was recorded in Sivasagar for all the four viruses. However, overall incidence of Begomovirus was low in all the surveyed fields.
A field experiment was conducted at Horticultural Research Station, Assam Agricultural University, Kahikuchi, Guwahati during 2009 to 2016 for evaluation of the performance of black pepper varieties/hybrids grown as mixed crop in 16 years old block of arecanut garden (Variety-Kahikuchi Local). Varieties such as, Panniyur-2, , Panniyur-4, Panniyur-5, Sreekara, Subhakara, Panchami, Pournami, PLD-2, Chumala, Karimunda and hybrids such as Panniyur-1 and Panniyur-3 were grown in three replications. Among the varieties /hybrids, Karimunda, Panniyur-5, Sreekara, Subhakara and Panchami produced higher number of laterals with more spread at six years age compared to the other varieties. Panniyur-5 recorded significantly higher number of spikes (210.3) followed by Karimunda (186.2); whereas the lowest was recorded in Panniyur-2 (62.8). The spike length was the highest in Panniyur-1 (16.4 cm), but was on par with Panniyur-1 and Subhakara. Sreekara recorded the lowest spike length of 10.2 cm. Three years mean dry berry yield indicated that , the yield was significantly higher with Panniyur-5 (1.25 kg/vine) followed by Panniyur-1 (1.15 kg/vine) and Subhakara (1.12 kg/vine) which differed significantly over the other varieties. Variety PLD-2 recorded higher oleoresin content (13.84%) and was on par with Panchami and Pournami. Piperine content was the highest in Panniyur-2 (4.65%) followed by Panniyur-1 (4.26%). Due to the mixed cropping of black pepper in arecanut garden, an improvement in the arecanut yield was observed (2.21 kg chali/ palm/year) during 2015-16 compared to the arecanut monocrop (1.96 kg chali/palm/year) during 2009-2010.
Background and Aim: The chicken gut harbors microflora which impacts the health, production performance and immune response against pathogens. Assam local chickens reared under natural conditions are known to possess high immunocompetence which may be attributable to its gut microbiota make-up. This study aimed to investigate the individual effect of two strains of Lactobacillus reuteri PIA16 isolated separately from cecum and jejunum of Assam indigenous chicken on the immunity of broiler chickens against Newcastle disease virus (NDV) when fed singly and in combination with a prebiotic. Materials and Methods: A total of 240 birds (48 per group) were vaccinated with Lasota strain of NDV on the 5th and 21st day of age. Blood samples were collected before and after immunization against ND for the detection of humoral antibody response by hemagglutination inhibition test. The cell-mediated immune (CMI) response was estimated through response to phytohemagglutinin-P (PHA-P) and expressed as web index. Results: A significant influence on the immune response to NDV was observed in all the L. reuteri PIA16 as well as mannan oligosaccharide (MOS) supplemented groups revealing higher antibody titer than the control counterpart. The CMI response revealed a better cutaneous basophilic hypersensitivity response to PHA-P in the treated groups than the control. Conclusion: Enhancement in immunity was perceived in the broilers fed with L. reuteri PIA16 and in combination with MOS due to the stimulation of the host's humoral and CMI response by the probiotics and prebiotics used. Keywords: hemagglutination inhibition, Lactobacillus reuteri PIA16, mannan oligosaccharide, Newcastle disease, phytohemagglutinin-P, probiotics.
Five hybrid brinjal varieties viz., Ketan, Vijay Kiron, Utkal Green, NV2035 Sarpan Bharata and two local cultivars viz., JC-1, Longai were evaluated at Horticultural Research Station, Kahikuchi, Guwahati as late rabi crop during 2014-16. Significant variation in plant growth parameters and fruit yield as well as on bacterial wilt incidence (Ralstonia solanacearum) were shown by all the varieties.Utkal Green recorded the highest per plant fruit yield (2.32 kg/plant) leading to the maximum total marketable yield (469.66 q/ha) and longer active fruiting duration (43.70 days).It also showed resistant reaction to bacterial wilt, recording only 12.44 per cent wilt incidence as compared to other varieties where it was in the range of 34.44 to 62.22 % at 55 days after transplanting. Although, Ketan and Vijay Kiron recorded moderately higher per plant fruit yield of 1.52 and 1.47 kg/plant,respectively but their total marketable fruit production reduced substantially due to high incidence of bacterial wilt (62.22 and 57.78%, respectively).The local cultivars, JC-1 and Longai exhibited moderately resistant reaction to bacterial wilt which registered 40.00 and 34.44 % , respectively.
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