Background: Infection involving the cerebrum is a true neurosurgical emergency that requires rapid diagnosis and appropriate surgical and medical intervention to achieve good clinical outcome.Methods: In this study we evaluated 41 pateints with brain abscess in our hospital in during 2005-2010. Demographic information, predisposing factors, clinical manifestations, lab. data and managements were evaluated.Results: In this study %53.6 of patients were 15-29 years old, %26 were 30-49 and %17 were 50-70 years old. Clinical manifestation was as follow in our study: Headache %92.6, nausea and vomiting %73.1, meningeal signs %17, drowsiness %12, decreased level of consciousness %9.7, fever %9.7, uninary and fecal incontinency % 7.3, visual disturbance % 7.3, chills %4.8. Predisposing condition leading to brain abscess in our study were sinusitis in %9.17, otitis in % 12, CSF rhinorrhea in % 2.4, mastoiditis in % 7.2, neurosurgery in % 17 and endocaiditis in %2.4. %20 of our patients had no risk factor. Antibiotics used in our study were ceftriaxne, metronidazole and vancomycin. Mortality rate was %12 in this study. % 9.7 of our patient that were admitted with diagnosis of brain abscess finally diagnosed as they had acute demyelinating encephalomyelitis (ADEM).
Conclusion:With promp diagnosis and treatment most cases of brain abscess survived. Few cases initially diagnosed as brain abscess may have other diagnosis like acute demyelinating encephalomyelitis (ADEM).
Background: Pleomorphic Acinetobacter baumannii is a highly successful nosocomial pathogen. It is an obligate aerobic, gram negative bacteria that causes nosocomial pneumonia and meningitis among hospitalized patients. Broad range resistance to multiple antibiotics has been the biggest challenge to treat A. baumannii infection. Here, we described the production, characterization, purification and identification of monoclonal antibody specific against the multi-drug resistant A. baumannii.Methods & Materials: BALB/c mices were immunized with formaldehyde-fixed A. baumannii M28-47 strain. The spleen was removed and fused with myeloma cells. A series of monoclonal antibodies (mAb) specific to A. baumannii were produced. Characterization of the mAb was performed using enzyme-linked immunosorbent assay (ELISA) with subclass specific goat antisera. Antibodies were purified by using the protein G-linked magnetic beads. SDS-PAGE and immunoblot were performed to confirm the presence of the heavy and light chain of IgG of the purified antibody. Mass spectrometry was used to further confirm the identity of the antibody. Immunoblotting was performed to detect the A. baumannii protein.Results: All the hybridoma clones produced IgG1 subclass antibodies. SDS-PAGE of the purified hybridoma antibody showed the presence of the antibody heavy chain and light chain. The presence was also confirmed by ELISA, immunoblot and mass spectrometry. All the purified antibodies detected A. baumannii protein prepared on nitrocellulose membrane from A. baumannii cell lysate.Conclusion: Monoclonal antibodies specific against A. baumannii was successfully prepared. This mAb could be useful as reagents for studying A. baumannii.
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