The Genotype-Tissue Expression (GTEx) project was established to characterize genetic effects on the transcriptome across human tissues and to link these regulatory mechanisms to trait and disease associations. Here, we present analyses of the version 8 data, examining 15,201 RNA-sequencing samples from 49 tissues of 838 postmortem donors. We comprehensively characterize genetic associations for gene expression and splicing in cis and trans, showing that regulatory associations are found for almost all genes, and describe the underlying molecular mechanisms and their contribution to allelic heterogeneity and pleiotropy of complex traits. Leveraging the large diversity of tissues, we provide insights into the tissue specificity of genetic effects and show that cell type composition is a key factor in understanding gene regulatory mechanisms in human tissues.
In laboratory and field bioassays, Gnathotrichus sulcatus responded to sulcatol (6-methyl-5-hepten-2-ol) only when both enantiomers were present. Response was greater to racemic sulcatol than to a mixture (65 : 35) of S-(+) and R-(-) enantiomers, the naturally occurring isomeric ratio. Enantiomer-specific active sites on receptor proteins in the same or different cells are implicated.
A mark–recapture study examined the spring flight dispersal of the ambrosia beetle, Trypodendron lineatum (Olivier), in an even-aged second-growth coastal forest in British Columbia. Pheromone-baited traps were placed in circular traplines at distances from 5 to 500 m from a central release location to (1) examine the relationship between wind direction and beetle catches in traps arranged around the release point, and (2) evaluate beetle catch characteristics when distances to baited traps were varied. A total of 29 800 marked beetles were released in three experiments. Upwind flight was most strongly exhibited at 5 m, with an upwind trend at 25 m, and no consistent flight pattern at 100 m, when wind movement was significantly directed. When the closest attraction was 100 m from the release point, beetles were caught uniformly in all directions indicating that flight was non-directional with respect to wind, for light wind speeds. Catches at 500-m traps tended to be downwind, thus beetles capable of flying that distance were ones that were flying with the wind. Equal numbers of beetles were captured at 5, 25, and 100 m despite increased intertrap spacings of 8, 20, and 32 m, respectively. A higher proportion of beetles were captured at 100 m when close-range traps at 5 and 25 m were not present.
The effects of trap design, lure concentration, lure age, and trap maintenance on the catch of western spruce budworm moths, Choristoneura occidentalis Freeman, in pheromone traps were tested in light to moderate infestations near Ashcroft, B.C. High cumulative moth catches reduced the trapping efficiency of both the sticky traps and the non-sticky Uni-traps relative to traps from which the moths were removed every 2 days. Correlations between the total season’s catch and larval density per plot in the same and following generations were not significant (P>0.05) for any of the eight combinations of trap design, lure concentration, and maintenance regimen tested. However, by dividing the mean moth catch in Uni-traps by either the basal area or foliage biomass per hectare in each plot, correlations with the following year’s larval density were significant (r2 = 0.77–0.98; P<0.05; n=5).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.