No abstract

No abstract

Piper solmsianum C.DC., which is popularly known as pariparoba, is a shrub that measures 1-3 m in height and it inhabits areas with wet tropical soils. The objective of this study was to analyze the leaf and stem anatomy using light microscopy, scanning electron micrographs, and energy-dispersive X-ray spectroscopy in order to provide information for species identification. The anatomical profile showed the following main microscopic markers: hypostomatic leaf; hypodermis layer on both sides; pearl glands; biconvex midrib shape; five collateral vascular bundles in open arc with the central bundle larger than the others; circular stem shape; collateral vascular bundles arranged in two rings; sinuous sclerenchymatic sheath in the pith; secretory idioblasts; and starch grains in the mesophyll, in the ground parenchyma of the midrib, petiole, and in the stem; and six morphotypes of calcium oxalate crystals (styloids, cuneiform, tabular crystal rosettes, cuneiform crystal rosettes, elongated square dipyramids, as well as very elongated square dipyramids).

No abstract

Aim of study: The aim of this study was to verify the differences in VIS/NIR spectra and leaf color parameters of leaves of Eucalyptus badjensis, E. benthamii, E. dunnii, E. grandis, E. globulus and E. saligna, at four ages, and their influence on species discrimination. Area of study: São Mateus do Sul, Paraná, Brazil.           Material and methods: Seedlings of the six species, with four replicates for each, were acclimatized in the same environment, in October 2015, in an entirely randomized design. Leaf samples were collected from plants that were 6, 8, 10 and 12 months old. Three leaves from each of four plants at each age were analyzed. Five parameters were recorded referring to the adaxial surface of each leaf, with a total of 15 records from repetitions and 60 per species at each age. The evaluation was performed in the spectral ranges from 360-740 nm (VIS) and 1000-2500 nm (NIR). Principal component analysis and linear discriminant analysis were performed. Main results: The influence of age differed within each species. In color data, the parameter with most variation among all samples was chromatic coordinate b*. In reflectance spectra (VIS), age of 12 months provided the best discrimination of species. Second derivative NIR spectra produced the best results of external prediction of Linear Discriminant Analysis models based on leaves of 12-month-old trees. Research highlights: Observation of color parameters and VIS/NIR spectroscopy have potential utility for discrimination of Eucalyptus species based on their green leaves.