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Bats represent the major source of human rabies cases in the New World. In the USA, most cases are associated with species that are not commonly found or reported rabid. To understand better the epidemiology and public health significance of potentially important bat species, a molecular study was performed on samples collected from naturally infected rabid western pipistrelle (Pipistrellus hesperus), eastern pipistrelle (Pipistrellus subflavus) and silver-haired bats (Lasionycteris noctivagans) from different regions of their geographical distribution in the USA. A 264 bp fragment at the 59 end of the N gene coding region was sequenced and analysed in comparison with rabies virus variants circulating within other North American mammals. Phylogenetic analysis demonstrated that P. hesperus bats maintain a unique rabies virus variant. Preliminary data also suggest that P. subflavus and Lasionycteris noctivagans may harbour two different rabies virus variants (Ps and Ln) that are likely to be maintained independently by each bat species, which recently appear to have emerged as major vectors of human disease. The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are DQ445308-DQ445382.

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e47614th International Congress on Infectious Diseases (ICID) Abstracts fied 19.2% (24/125) more positive cases, 50% (12/24) were HPV18, 37.5% (9/24) HPV16 and in 12.5% (3/24) amplification of more than one genotype occurred. Conclusion:In conclusion, target region for amplification of HPV DNA is a determinant factor in the molecular diagnosis of cervical infection. Moreover, quantification of the viral load by qRT-PCR SYBR Green-L1 assay allows to determines HPV infection, viral load, genotyping especially in women with high risks lesions by PAP smear.

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