Ensaios de investigação molecular para diagnóstico da tuberculose bovina têm sido amplamente estudados. Estes métodos vêm possibilitar maior agilidade e possuem alta sensibilidade e especificidade além de permitirem realização de diagnóstico diferencial entre doenças e a identificação precisa do agente etiológico, sendo de grande importância para a epidemiologia, tratamento e prognóstico especialmente na saúde pública. Neste contexto a de extração de DNA tem sido apontada como etapa limitante ao uso da PCR em amostras clínicas devido à complexidade da parede celular micobacteriana e dificuldade de purificar a bactéria dessas amostras. O trabalho objetivou avaliar protocolo de extração de DNA de Mycobacterium, coletados diretamente do granuloma de bovinos com suspeita de tuberculose abatidos em frigoríficos da Bahia. Foram submetidas ao protocolo de extração para bactérias gram-positivas (QIAGEN, Alemanha) adaptado, 74 lesões resultando em excelentes concentrações e elevado grau de pureza do DNA. Os resultados indicam que o protocolo utilizado mostrou-se eficaz.
The diagnosis of bovine tuberculosis (TB) by molecular techniques has been broadly studied. These methods allow accelerating the diagnosis, in addition to presenting high specificity and sensitivity in the identification of the pathogen, critical characteristic for public health, especially when it comes to the direct diagnosis of the biologic samples, which has been little explored. This paper has evaluated a multiplex polymerase chain reaction (mPCR) as a tool to diagnose TB, which was performed directly on the granulomatous material of suspicious lesions collected in a cold chamber under state inspection in the state of Bahia, Brazil. Of the 74 samples evaluated, 14.86% were positive, with 10.81% positive for mPCR and culture, 4.05% negative for cultivation and positive for mPCR. The correlation between the cultivation and the mPCR presented agreeance higher than 61.54% of the cases. The results have indicated that the protocol proved itself effective, fast and very promising in the surveillance in slaughterhouses for the diagnosis of tuberculosis directly from the granuloma.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.