Background: Traumatic lesions, whether chemical, physical, or thermal in nature, are among the most common lesion in the mouth. Wound healing is essential for the maintenance of normal structure, function, and survival of organisms. Experiments of Robusta coffee powder on rat-induced alloxan incision wound, clinically demonstrated similar healing rate with the povidone iodine 10%. No studies that look directly the effect of coffee extract in ointment form when viewed in terms of histopathology. Robusta coffee bean (Coffea canephora) consists of chlorogenic acid (CGA) and caffeic acid which are belived to act as antioxidant and take part in wound healing process. Purpose: The aim of this study was to identify the enhancement of healing process of full-thickness skin wound after Robusta coffee beans extract ointment application. Methods: Sample consisted of 20 Cavia cabaya treated with full-thickness with wounds and was given Robusta coffee beans extract ointment concentration range of 22.5%, 45%, and 90% except the control group which was given ointment base material. Animals were then harvested on the fourth day and made for histopathological preparations. Data were calculated and compared by one-way ANOVA test and LSD test. results: The study showed that Robusta coffee bean extract ointment can increase the number of lymphocytes, plasma cells, macrophages, fibroblasts, and blood vessels by the presence of chlorogenic acid (CGA) and Caffeic acid. Conclusion: In conclusion Robusta coffee bean extract ointment enhance the healing process of fullthickness skin wound of Cavia cabaya.Key words: Robusta coffee bean extract, the healing process, chlorogenic acid, caffeic acid abstrak latar belakang: Lesi traumatik, baik akibat rangsang kimia, fisik, atau termal, merupakan lesi yang paling umum terjadi di dalam rongga mulut. Penyembuhan luka yang terjadi ini penting untuk pemeliharaan struktur normal, fungsi, dan kelangsungan hidup organisme. Percobaan pemberian bubuk kopi Robusta terhadap luka sayatan pada tikus yang diinduksi aloksan, secara klinis menunjukkan tingkat penyembuhan yang sama dengan povidone iodine 10%. Namun belum ada penelitian yang melihat secara langsung pengaruh ekstrak kopi dalam bentuk salep bila dilihat dari segi histopatologi. Biji kopi Robusta (Coffea canephora) terdiri dari chlorogenic acid (CGA) dan caffeic acid yang dipercaya berperan sebagai antioksidan dan mengambil bagian dalam proses penyembuhan luka. tujuan: Tujuan penelitian ini adalah untuk mengidentifikasi peningkatan proses penyembuhan luka full-thickness pada kulit setelah pengaplikasian salep ekstrak biji kopi Robusta. Metode: Sampel terdiri dari 20 Cavia cabaya yang diberi perlakuan berupa luka sayat full-thickness pada kulit punggung dan diberi salep ekstrak biji kopi Robusta dengan beberapa konsentrasi, yaitu 22,5%, 45%, dan 90%, sedangkan kelompok kontrol hanya diberi bahan dasar salep. Binatang coba kemudian dieksekusi pada hari keempat dan dibuat sediaan histopatologinya. Data dihitung dan dibandingkan dengan uji One-Way ANO...
Background: Ubiquinone is one of food supplement which is known have positive effect in wound healing. However the study to evaluate the possible role of ubiquinone in bone healing in autogenous bone grafting after mandibular resection has not been studied. An in vitro study is required to evaluate whether ubiquinone or coenzyme Q-10 (CoQ10) has a positive effect on osteogenesis. Viability test of CoQ10 and a model of osteogenic-induced and hypoxic-condition mesenchymal stem cell culture were established to support the study. Purpose: The study was made to evaluate the role of ubiquinone in osteogenesis by analyzing the toxicity effect and the optimal dose of CoQ10 that might interfere in bone marrow derived mesenchymal stem cell (BM-MSC) that was dose in cell culture medium. The BM-MSC culture under hypoxia condition were also observed. Method: The toxicity and the optimum viability concentration of ubiquinone were observed using MTT assay. The osteogenic differentiation under hypoxic condition was done on BM-MSC in osteogenic medium that composed of ascorbic acid, glycerolphosphate and dexamethasone in hypoxia chamber for 21 days. Osteogenic differentiation and cellular hypoxia features were tested with immunocytochemical staining using anti-Runx2 and anti-HIF1α monoclonal antibody, respectively. Result: the maximum density value of 1.826 was found in the group of ubiquinone concentration of 75μM/ml, increasing of in concentration of ubiquinone resulted with the decrease of optical density of CoQ10. Statistic analysis using Anova showed with no significant difference among groups with various concentration. Immunocytochemical staining showed that Runx2 expression in 3% hypoxia group (p<0.05). Conclusion: Ubiquinone was found non toxic in its optimum dose of 75μM/ml, showed by optimum result in the expression of Runx2 and HIF1α further study is necessary to evaluate the angiogenic and osteogenic effect of ubiquione.
Background: The presence of cell or tissue damage always followed byinflammatory reactions. Inflammatory process should not persist because it can interfere even inhibit regeneration. An increased circulation of IL-1, IL-6 and TNF-alpha happens in chronic inflammation. IL-1 is granulocytes chemoattractant and has function to localize inflammation. Overexpression of IL-1 can inhibit wound healing so it needs currative treatment to decrease IL-1's expression. Purpose: To know expression of IL-1 after provision of Clariasbatrachus fish oil. Methods: We have used the post test only design in this research. There have been 19 Rattusnovergicus as research samples and those were divided into 3 groups, group 1 as control, group 2 has given 10% fish oil and group 2 has given 5% fish oil. Chronic inflammation treated to all samples for three days. The group 1 and 2 given Clariasbatrachusfish oil on the third day. We did decapitation on the fourth day to perform Immunohistochemistrystaining. The data were tabulated and analyzed statistically used Oneway ANOVA. Result: Group 2 and 3 has lower IL-1 expression than Control group.There is significant difference between groups. Conclusion: Clariasbatrachus Fish oil can lowered IL-1 expression onmucosal cavity's chronic inflammation.
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