Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient

Wati, Sisca Meida; Istiati, Istiati; Soesilawati, Pratiwi

doi:10.20473/j.djmkg.v47.i3.p141-145

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…IL-1 berperan dalam menginduksi PMN (polymorphonuclear) untuk bergerak menuju daerah infeksi, sedangkan TNF-α berperan dalam menghambat apoptosis dari PMN, yang mengakibatkan meningkatnya infiltrasi PMN dapat dilihat pada setiap tahap periodontitis. 4 Metronidazole merupakan salah satu drug of choice untuk penyakit periodontal. Metronidazole efektif terhadap bakteri anaerob seperti P.gingivalis.…”

Section: Pendahuluanunclassified

Potensi ekstrak daun singkong (Manihot esculenta Crantz.) terhadap profil leukosit darah tepi model tikus disfungsi ovarium dan periodontitisPotential of cassava leaves (Manihot esculenta Crantz.) extract on peripheral blood leukocyte profile in ovary dysfunction and periodontitis rat model

et al. 2021

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Pendahuluan: Disfungsi ovarium merupakan keadaan ovarium yang mengalami kegagalan dalam sekresi hormon seks steroid. Penurunan sekresi hormon terutama estrogen dapat memicu peningkatan sitokin proinflamasi seperti IL-1, IL-6 dan TNF-α yang berperan dalam resorpsi tulang dan reaksi inflamasi periodontal. Salah satu drug of choice dari periodontitis adalah metronidazole. Penggunaan metronidazole secara sistemik dapat mengakibatkan efek samping, sehingga diperlukan bahan alternatif yang memiliki efek terapi antiinflamasi. Salah satu tanaman yang memiliki efek antiinflamasi adalah daun Singkong. Tujuan penelitian adalah menganalisis potensi ekstrak daun Singkong sebagai antiinflamasi terhadap profil leukosit darah tepi model tikus disfungsi ovarium dan periodontitis. Metode: Jenis penelitian eksperimental laboratoris dengan post-test only control group design. Sampel penelitian sebanyak 18 ekor tikus betina yang dibagi menjadi dua kelompok besar yakni kelompok disfungsi ovarium dan kelompok kecil periodontitis. Pembuatan model tikus disfungsi ovarium dan tikus periodontitis dilakukan selama 28 hari. Setelah masing-masing kelompok dibagi menjadi 3 kelompok yang diberikan perlakuan aquades, metronidazole dan ekstrak daun Singkong selama 7 hari. Setiap tikus diambil darahnya melalui plexus infraorbitalis pada hari ke-1, hari ke-3 dan hari ke-7. Darah yang diambil selanjutnya dilakukan perhitungan jumlah sel leukosit darah tepi menggunakan kamar hitung dan perhitungan jenis dengan cara visual. Hasil: Hasil uji LSD menunjukkan bahwa terdapat perbedaan bermakna jumlah leukosit darah tepi antar hari pada kelompok tikus yang mengalami disfungsi ovarium (p≤0,05). Simpulan: Ekstrak daun Singkong (Manihot esculenta Crantz) dapat menurunkan profil leukosit perifer model tikus disfungsi ovarium dan periodontitis.Kata kunci: Disfungsi ovarium, periodontitis, profil leukosit perifer, ekstrak daun singkong. ABSTRACTIntroduction: Ovarian dysfunction is when the ovaries fail in the secretion of steroid sex hormones. Decreased secretion of hormones, especially estrogen, can trigger an increase in pro-inflammatory cytokines such as IL-1, IL-6 and TNF-α, which play a role in bone resorption and periodontal inflammatory reactions. One drug of choice for periodontitis is metronidazole. Systemic use of metronidazole can cause side effects, so an alternative material with a therapeutic anti-inflammatory effect is needed. One of the plants that have an anti-inflammatory effect is cassava leaves. The research objective was to analyse the potential of cassava leaf extract as an anti-inflammatory against the peripheral blood leukocyte profile in a mouse model of ovarian dysfunction and periodontitis. Methods: This was an experimental laboratory study with a post-test only control group design. The research sample consisted of 18 female rats divided into two large groups: the ovarian dysfunction group and the small periodontitis group. The making of mouse models of ovarian dysfunction and periodontitis rats was carried out for 28 days. After each group was divided into three groups treated with distilled water, metronidazole and cassava leaf extract for seven days. Blood was drawn from each mouse through the infraorbital plexus on day 1, day three, and 7. The blood that was taken was then performed to calculate the number of peripheral blood leukocytes using the counting room and the calculation of the type by visual means. Results: The results of the LSD test showed that there was a significant difference in the number of peripheral blood leukocytes between days in the group of rats with ovarian dysfunction (p≤0.05). Conclusion: Cassava leaf extract (Manihot esculenta Crantz) can reduce the profile of peripheral leukocytes in a mouse model of ovarian dysfunction and periodontitis.Keywords: Ovarian dysfunction, periodontitis, peripheral leukocytes profile, cassava leaf extract.

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Section: Pendahuluanunclassified

Potensi ekstrak daun singkong (Manihot esculenta Crantz.) terhadap profil leukosit darah tepi model tikus disfungsi ovarium dan periodontitisPotential of cassava leaves (Manihot esculenta Crantz.) extract on peripheral blood leukocyte profile in ovary dysfunction and periodontitis rat model

et al. 2021

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“…Empat belas subyek (n=14) yang terpilih sesuai dengan rumus ukuran sampel untuk menguji perbedaan dua rata-rata data tidak berpasangan, plak supragingival dibersihkan dan gigi diisolasi menggunakan cotton roll agar tidak terkontaminasi oleh saliva, dilakukan pengambilan cairan sulkus gingiva menggunakan metode absorbing paper strip dengan cara intracrevicular yaitu paper strip dimasukkan kedalam sulkus gingiva sampai ke dasar sulkus dan didiamkan selama 30 detik. 27 Penelitian ini menggunakan paper point no.30 27 dalam waktu 2-3 menit sehingga didapatkan 6-8 paper point dalam satu sisi poket, kemudian dimasukkan ke dalam tabung yang sudah diisi dengan phosphat buffer dengan perbandingan 1:1000 ml sebagai pelarut, selanjutnya dilakukan tindakan skeling dan root planing menggunakan ultrasonic scaler, setelah tindakan diberikan aplikasi gel teh hijau seperti pada gambar 1 sebagai terapi tambahan dengan cara dimasukan ke dalam sulkus gingiva sampai meluap kearah korona, dikategorikan kedalam kelompok uji dan dihitung sebagai hari ke-0. Gigi berseberangan yang memiliki kedalaman poket ≥ 5 mm dilakukan tindakan skeling dan root planing, namun tidak diaplikasikan gel teh hijau dan dikategorikan ke dalam sisi kontrol dan dihitung sebagai hari ke-0.…”

Section: Metodeunclassified

Pengaruh aplikasi gel teh hijau (Camellia sinensis) terhadap kadar total antioxidant capacity (TAOC) sebagai perawatan tambahan dari skeling dan root planing pada pasien dengan periodontitis kronisEffect of green tea (Camellia sinensis) gel application on the total antioxidant capacity (TAOC) levels as adjunct to scaling and root planing in patients with chronic periodontitis

2020

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Pendahuluan: Mekanisme peradangan periodontitis kronis menghasilkan reaksi oksidasi, diketahui melalui kadar total antioxidant capacity (TAOC) cairan sulkus gingiva. Teh hijau dapat menghambat bakteri patogen periodontal sehingga kerusakan jaringan periodontal tidak bertambah parah. Tujuan penelitian adalah menganalisis kadar TAOC sebelum dan sesudah skeling dan root planing disertai gel teh hijau sebagai antioksidan. Metode: Jenis penelitian eksperimen semu, rumus ukuran sampel untuk menguji perbedaan dua rata-rata data tidak berpasangan, setiap subyek memiliki poket ≥ 5 mm, dikelompokkan sisi uji (n=14) dan sisi kontrol (n=14) (split mouth). Pengambilan cairan sulkus gingiva dilakukan pada hari ke-0, 15, dan 30, menggunakan metode absorbing paper strip dengan cara intracrevicular. Kedua sisi dilakukan skeling dan root planing, sisi uji diberikan gel. Hasil: Perbandingan kadar TAOC antara sisi kontrol dan sisi uji pada hari ke-0 (p=0,986), hari ke-15 (p=0,836), dan hari ke-30 (p=0,371) menunjukkan tidak terdapat perbedaan bermakna (p-value>0,05). Perbandingan rerata selisih kadar TAOC dalam kedua kelompok antara hari ke-0 dengan ke-15 (p=0,946), hari ke-0 dengan hari ke-30 (p=0,504), serta hari ke-15 dengan hari ke-30 (p=0,811) jugwa menunjukkan tidak terdapat perbedaan bermakna. Simpulan: Tidak terdapat pengaruh aplikasi gel teh hijau (Camellia sinensis) terhadap kadar total antioxidant capacity (TAOC) pada perawatan periodontitis kronis berupa skeling dan root planing.Kata kunci: Gel teh hijau, periodontitis kronis, skeling dan root planing, total antioxidant capacity (TAOC). ABSTRACTIntroduction: The inflammatory mechanism of chronic periodontitis produces an oxidation reaction, measured through the level of total antioxidant capacity (TAOC) of the gingival crevicular fluid. Green tea can inhibit periodontal pathogenic bacteria so that periodontal tissue damage will not worsen. This study was aimed to analyse the levels of TAOC before and after scaling and root planing with green tea gel as an antioxidant. Methods: The research was quasi-experimental. The sample size formula was used to test the difference between two unpaired data means. Each subject has a pocket ≥ 5 mm, grouped by the test side (n = 14) and the control side (n = 14) (split-mouth). The gingival crevicular fluid was collected on days 0, 15, and 30, using the intracrevicular method with absorbing paper strips. Both sides were treated with scaling and root planing, and the test side was administered with the green tea gel. Results: Comparison of TAOC levels between the control side and the test side on day 0 (p = 0.986), day 15 (p = 0.836), and day 30 (p = 0.371) showed no significant difference (p-value > 0.05). Comparison of the mean difference of the TAOC levels in two groups between day 0 with day 15 was p = 0.946, day 0 with day 30 was p = 0.504, and day 15 with day 30 was p = 0.811; which also showed no significant difference. Conclusion: There is no effect of green tea gel (Camellia sinensis) application on the Total Antioxidant Capacity (TAOC) level in chronic periodontitis treatment in the form of scaling and root planing.Keywords: Green tea gel, chronic periodontitis, scaling and root planing, total antioxidant capacity (TAOC).

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“…19 The specific bacteria that cause periodontitis are Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia. 20,21 Periodontitis is now classified based on the staging and grading method. Periodontitis is diagnosed by looking at the signs of changes on the gingiva and the attachment loss accompanied by periodontal pockets.…”

Section: Introductionmentioning

confidence: 99%

Periodontal disease severity in patients with long COVID and non-COVID-19

Louisa,

Amalina,

Putranto

et al. 2024

Dent. J.

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Background: Previous research studies have found the persistence of various COVID-19 symptoms even after the patient tested negative on a PCR test; this incident is now known as long COVID. These long COVID symptoms are reported to appear in the oral cavity including long COVID effects on periodontal disease, as both long COVID and periodontal disease release similar proinflammatory cytokines such as Acute phase proteins, CRP, TNF-α, IL-1β, IL-2, IL-6, and IFN-g. Purpose: This study aims to show periodontal-disease severity-frequency distribution in COVID-19 survivors with long COVID and in non-COVID-19 patients. Methods: Patients’ secondary data in the Periodontics Clinic Faculty of Dentistry at Trisakti University Dental Hospital (n=40) consisted of 20 samples from COVID-19 survivors who experienced long COVID and 20 samples from the non-COVID-19 group selected according to the inclusion criteria. Afterward, the data was recapitulated and processed into a research report. Results: The distribution percentage of generalized gingivitis was highest in non-COVID-19 patients, while generalized periodontitis was highest in COVID-19 survivors with long COVID. Based on periodontitis staging and grading methods, it is not proven that long COVID increases the severity of the periodontitis. Conclusion: This research shows that the distribution of gingivitis in COVID-19 survivors with long COVID has not increased. Meanwhile, the distribution of general periodontitis increased in survivors with long COVID. However, there was no increased severity of periodontitis based on the staging and grading method of periodontitis in the COVID-19 survivors with long COVID.

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Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient

Cited by 3 publications

References 10 publications

Periodontal disease severity in patients with long COVID and non-COVID-19

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