No abstract

Esse trabalho foi desenvolvido com o objetivo de caracterizar – por meio de um portal de visualizações - o panorama e os impactos da atuação feminina na computação no Brasil. Essa iniciativa engloba um escopo nacional, com análise acadêmica e mercadológica, e apresenta como produto final um portal que possibilita e facilita o compartilhamento informação e a disseminação de conhecimento relacionado a atuação da mulher brasileira na computação.

Introduction: Lipids are molecules that stand out among the different cellular metabolites by their enormous molecular diversity. Their functions were initially related to the composition of biological membranes and energy storage, but currently, these molecules have been analyzed considering different functions and regulatory signaling (Loizides-Mangold, 2013). It is known that lipid membranes and lipid mediators constitute specific phenotypes, including tumors (Hilvo et al, 2011). Lipid metabolism in cancer had been studied predominantly at the genetic level has recently gained further interest. Lipidomics studies show a powerful means of investigating pathophysiological issues and involvement of lipids in pathological states for both diseases in which lipids are known to play a role, but also for those which role is not well characterized (Roberts et al., 2008). Myeloid neoplasms are clonal diseases of the hematopoietic stem cell which can be present in the bone marrow and/or peripheral blood. Over the past two decades, mass spectrometry (MS) has emerged as the main method used in lipidomics analysis, which allows the structural characterization and quantification of complex lipids and their metabolites (MURPHY et al, 2005). Due to the importance of this field we have considered the use of the lipidomic innovative platform to identify differences in the plasma lipid metabolomic profile of hematological patients with Myeloid Neoplasms. Methods: Untargeted Shotgun MS/MS Analysis was performed on an independent service at the AB-Sciex Laboratory located in Sao Paulo, SP, Brazil on a 5600 Triple TOF mass spectrometer (ABSciex) instrument with an acquisition scan rate of 100 spectra/sec and stable mass accuracy of ~2 ppm. Plasma samples from 153 participants were analyzed being, 90 of the Control Group, 43 Myeloproliferative Neoplasms (MPN), 11 Myelodysplastic Syndromes (MDS) and 9 Acute Myeloid Leukemias (AML). Data were acquired using the AB-Sciex Analyst TF, processed using the AB-Sciex LipidViewTM and the web-based analytical pipeline MetaboAnalyst 2.0 (www.metaboanalyst.ca) (Xia et al, 2012). Results: Untargeted analysis identified in negative and positive-modes a total of 658 features at 2 ppm resolution. PCA and PLS-DA analysis revealed clear discrimination among groups, in particular for AML patients. Main lipid groups differentially expressed were: Monoacylglycerols (MAG), Glucosylceramide E (GlcdE), Ethyl Esters (EE), Lysophosphatidic acid (LPA), Sulfoquinovosil diacylglycerols (SQDG), Monoglycerols (MG), Methyl Ethanolamines (ME), Lysophosphatidylcholines (LPC), Dimethyl Phosfatidyletanilamines (DMPE), Monometylphosphatidiletanolamines (MMPE), Ceramide-1-phosphate (CerP), Glicerophosphoglycerols (GP), Lysomonomethyl Glycerophosphocholine (LMMPE), Phosphatidic Acids (PA), Ergosterols (ERG), Glycerophosphoserine (PS), Diacylglycerols (DAG), Hexocylceramides (HexCer) and Lanosterol (Lan). ROC Curve Analysis revealed Total LMMPE as the strongest discriminating marker between Controls from Patients with MDS or AML (Sensitivity= 0.95 (0.824-1); Specificity= 0.8941 (0.847-0953); Positive Likelihood Ratio= 8.972 and Negative Likelihood Ratio =0.05592 and T Test= 7.576E-12). In addition these lipids were also able to differentiate MDS and AML from NMP (Sensitivity= 0.9118 (0.824-1), Specificity= 0.95 (0.85-1), Positive Likelihood Ratio= 18.2 and Negative Likelihood Ratio= 0.05592). Conclusions: The Myeloproliferative Neoplasms from the point of view of global plasma lipidomics are accompanied by several modifications. In particular the Lysomonomethyl-Phosphatidylethanolamines (LMMPE) seems to play important differentiating roles among them. Disclosures No relevant conflicts of interest to declare.

No abstract

No abstract

O presente artigo se dedica ao estudo do conceito de teodiceia que emerge da construção da obra O Senhor dos Anéis, produzida originalmente por J. R. R. Tolkien. Buscou-se comparar a teorização de Collin Campbell quanto a uma suposta mudança da teodiceia ocidental, que estaria sendo influenciada na cultura e na religião para aderir a um conceito epistemologicamente oriental. Para tanto, se utilizou a análise fílmica e do discurso para abordar três cenas da obra. Percebeu-se que, apesar da aparente possibilidade de uma pluralização na construção teodiceica, não é possível acusar Tolkien ou Jackson de fugirem a uma tradicional construção de teodiceia a partir da perspectiva ocidental. Entretanto, é plausível argumentar que, apesar da construção da narrativa em si não aparentar traços orientalistas, mostra interessantes perspectivas e desdobramentos da presente discussão

No abstract

The objective of this study was to examine potential mechanisms leading to the often‐observed Vitamin B12 deficiency following Roux‐en‐Y gastric bypass (RYGB). Vitamin B12 absorption begins with release from food in the upper GI tract followed by transcobalamin I binding; duodenal enzyme degradation of transcobalamin I is then followed by intrinsic factor (IF) capture; IF is next degraded within the ileal enterocyte, releasing B12 that then enters the plasma. Although IF levels are reduced with RYGB due to removal of the gastric fundus, other causes of modified B12 transport may be involved. To examine these other potential mechanisms we used balloon endoscopy to acquire GI tissue at multiple sites in 20 obese women with adult‐onset diabetes before (age, 46.5±6.6 yrs; BMI, 45.7±4.1 kg/m2) RYGB and at the identical India‐Ink marked sites three months following surgery (BMI, 37.7±3.4 kg/m2). Microarray analysis was performed on all samples using a Human GeneChip 1.0 ST array (Affymetrix, Inc., Santa Clara, CA). There was significantly reduced gene expression (2.335‐fold, p<0.05) of TCN1 in the remnant stomach following surgery (TCN1 translates transcobalamin I). These findings suggest that B12 deficiency following RYGB may be caused by a reduction in transcobalamin I in addition to the anatomical absence of IF.FAPESP 2011/09612‐3, BEPE 2014/07742‐5.