Alliums are the most popular for their culinary usage and nutraceutical benefits. Their productions are greatly affected by the multiple biotic and abiotic stresses. Poor characterizations of genetic resources are the major bottleneck in genetic improvement of alliums. Chloroplast derived simple sequence repeat (cpSSR) have recently gained much popularity due to their maternal inheritance and low recombination along with their hypervariable nature. In this study, 22 chloroplast-derived SSR markers were produced from chloroplast genomes of A. cepa and A. sativum. Repeat comparison revealed tri nucleotide repeats were in higher proportion (50%) compared to other repeat motifs. The number of alleles ranged from 2 to 4, heterozygosity from 0.009 to 0.540, and PIC from 0.007 to 0.427. The polymorphism survey and clustering of twenty-two cpSSR markers of twenty-five alliums, lead to three groups (groups I, II, and III), indicated the usefulness of these cpSSR markers. This demonstrated that cultivated A. cepa and A. sativum belong to different groups II than most wild alliums, confirming the usefulness of the AccpSSR and AscpSSR markers that will allow introduction of desirable biotic and abiotic tolerance traits from various wild alliums to selected cultivated alliums. In addition, these cpSSRs were validated in 79 alliums, divided them into three groups using Jaccard dissimilarity and Bayesian model-based structure analysis. Subsequent clustering allowed us to identify diverse alliums, for constructing core collection of germplasm resource. The study will be useful for molecular breeding and genomic selection based crop improvement.
The persistence pattern and risk assessment of profenofos and triazophos in okra (Abelmoschus esculentus (L.) Moench) and cropped soil were studied in the present study. The insecticides were applied twice at 10 days interval @ 500 and 1000 g a.i./ha. Residues were quantified at 0, 1, 3, 5, 7, 10, 15 and 20 days of second application using GC equipped with Flame Photometric Detector (FPD). The average initial deposits of triazophos (1.838 mg/kg) in okra fruits were higher than profenofos (1.418 mg/kg) at the recommended application rates. Both the insecticides followed a first order kinetics with half-lives of 1.6 and 1.4 days, respectively on okra fruits. The initial deposits of 0.483 and 0.500 mg/kg, for respective insecticides, disappeared to BDL on 5th and 7th day in okra cropped soil. Microwave oven cooking proved more promising than other household processing in dislodging the residues of test insecticides from okra fruits. On the basis of this study, waiting periods of 7.6 and 7.4 days were suggested for consumption of okra sprayed with profenofos and triazophos, respectively.
This study documents the residual persistence of profenofos and triazophos on capsicum fruits. The persistence was observed on 0, 1, 3, 5, 7, 10, 15 and 20 days of insecticides spray and analyzed using QuEChERS technique and estimated with Gas Chromatograph equipped with Flame Photometric Detector. The analysis revealed the presence of profenofos with less initial deposits of 1.403 mg/kg compared to 1.870 mg/kg of triazophos which further reduced to below detectable level (BDL) on 15th and 10th day with residual half-lives of 2.0 and 1.5 days, respectively. The different household processing (s) from washing to cooking gave residual relief up to 73.77%. The soil analysis revealed initial deposits of 0.913 and 0.816 mg/kg, for respective insecticides, which disappeared to BDL on 10th day. The waiting periods worked out at the limit of quantification (0.05 mg/kg) were 10 and 8 days, for profenofos and triazophos, respectively.
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