Kepel (Stelechocarpus burahol) is an original Indonesian fruit plant. Kepel is currently hard to find and cultivate conventionally. In vitro culture is a method for kepel propagation. The in vitro culture requires an established protocol for explant sterilization to be successful. For the present, there is no appropriate kepel sterilization method that has been known. This study aimed to obtain a sterilization method for kepel embryo explants appropriate for embryo culture. This research was conducted in September 2018 - January 2019 at the In Vitro Culture Laboratory, Faculty of Agriculture, Universitas Muhammadiyah Yogyakarta. This study used an experimental method arranged in a single factor. Completely Randomized Design with the treatment of various immersion methods of kepel embryo explants divided into 8 levels. They are Sodium hypochlorite (NaOCl) (5% : 5', 5% : 10', 10% : 5', 10% : 10'), Hydrogen peroxide (H2O2) (2% : 10', 2% : 15', 3% : 10', 3% : 15') and plant growth regulators 2 ppm 2,4-D + 0,5 ppm BAP, 2 ppm NAA + 0,5 ppm BAP. The results showed that 5% NaOCl for 5 minutes treatment was the best method for sterilization with a percentage of viable explant 88.89%. Then, the percentage of browning 11.11% and 2 ppm 2,4-D + 0,5 ppm BAP was effective in the organogenesis of callus of kepel embryos.
Kepel (Stelechocarpus burahol [Bl] Hook F. & Th.) merupakan buah asli Indonesia berbiji banyak dengan ukuran yang besar. Bagian buah yang dapat dikonsumsi hanya 49% dengan bagian lain berupa biji. Perbanyakan kepel secara konvensional masih sulit dilakukan dengan hasil yang rendah. Kultur endosperma secara in vitro adalah metode perbanyakan yang tepat untuk memperoleh tanaman triploid dengan buah tanpa biji. Sterilisasi merupakan tahap awal yang menjadi kunci keberhasilan kultur in vitro. Hingga kini belum dilaporkan metode sterilisasi endosperma kepel secara in vitro yang tepat. Penelitian ini bertujuan untuk memperoleh metode sterilisasi eksplan yang tepat untuk kultur endosperma kepel. Penelitian ini telah dilaksanakan pada bulan September 2018 – Januari 2019 di Laboratorium Kultur In Vitro, Fakultas Pertanian, Universitas Muhammadiyah Yogyakarta. Penelitian ini menggunakan metode eksperimen disusun dalam Rancangan Acak Lengkap (RAL) faktor tunggal dengan perlakuan berbagai konsentrasi bahan sterilan yang terbagi 8 aras: H2O2 (3%10’, 3%15’, 5%10’, 5%15’) NaOCl (5%5’, 5%10’, 10%5’, 10%10’) dengan 3 kali ulangan dan 3 sempel. Parameter yang diamati yaitu: persentase kontaminasi, browning, hidup, vitrifikasi, jenis kontaminasi, waktu kontaminasi dan waktu browning. Hasil penelitian diperoleh bahwa perlakuan NaOCl 10% selama 10 menit merupakan metode sterilisasi paling tepat dengan presentase ekplan hidup 44,44%, persentase eksplan vitrifikasi 66,66%, serta tidak terjadi kontaminasi dan browning
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.