Optimasi Sterilisasi Endosperma Kepel (Stelecthocarpus burahol [Bl] Hook F. &amp; Th) Secara In Vitro

Handayani, Etty; Irsyadi, Muhammad Burhanuddin; Aris, Irfan; Alawiyah, Riffa Leshia Muhvi Nur; Ayuningtias, Nandini; Permatasari, Fany; Rineksane, Innaka Ageng

doi:10.32938/jbe.v6i2.1179

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…Menurut Handayani et al (2021) browning terjadi karena senyawa fenolik yang keluar dari eksplan akibat perlukaan yang mengaktifkan enzim polifenol oksidase (PPO) dan teroksidasi oleh oksigen.…”

Section: Persentase Eksplan Browningunclassified

In vitro shoot induction from petiole explants of tembesu (Fagraea fragrans Roxb) using 6-benzylaminopurine

Isda,

Afrilla

2023

J. Ilm. Pertan.

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Tembesu (Fagraea fragrans Roxb) is a valuable woody plant from the Loganiaceae family, utilized in construction and furniture production. Unfortunately, the tembesu population continues to decline due to excessive logging, forest fires, and a lack of cultivation efforts. Planting through seeds takes a long time, making in vitro propagation an attractive alternative. This research aims to evaluate the effect of 6-benzylaminopurine (BAP) in inducing shoots and determine the optimal BAP concentration on tembesu petiole explants in vitro. A completely randomized design (CRD) with six treatment levels (0, 0.5, 1, 1.5, 2, and 2.5 mg/L BAP) and five replications was employed. Despite a 10% shoot formation rate for each treatment, the optimal BAP concentration for callus formation was found to be 0.5 mg/L, with the highest percentage reaching 70%. The results indicate that adding different concentrations of BAP to the Murashige & Skoog (MS) medium was ineffective in inducing shoots in tembesu petiole explants. Therefore, further research and the development of more sophisticated in vitro propagation techniques are necessary to enhance the success of shoot induction in tembesu. It is hoped that this research will serve as a foundation and motivation for subsequent in-depth studies on the in vitro propagation of tembesu, supporting the conservation and development of this plant.

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Section: Persentase Eksplan Browningunclassified

In vitro shoot induction from petiole explants of tembesu (Fagraea fragrans Roxb) using 6-benzylaminopurine

Isda,

Afrilla

2023

J. Ilm. Pertan.

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“…However, in vitro propagation of Kepel plants still faces obstacles, including the browning of explants. Research on in vitro cultures of Kepel nodes by Sekar et al(2023), Hutabarat et al(2022), and S. burahol seed endosperm explants by Handayani et al (2021) showed that although callus induction in explants can be produced, browning is still one of the inhibiting factors in callus regeneration, resulting in a low percentage of S. burahol live callus.…”

Section: Introductionmentioning

confidence: 99%

Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

Sibarani,

Restiani,

Prasetyaningsih

2024

JPBN

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Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low explant in vitro regeneration of Kepel. Using nodal explant, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, and dark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest broning intensity, and the highest percentage of callus. This present study used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel node explants during 28 days of culture. The results of this study are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation. Keywords: Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol

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Optimasi Sterilisasi Endosperma Kepel (Stelecthocarpus burahol [Bl] Hook F. & Th) Secara In Vitro

Cited by 2 publications

References 2 publications

In vitro shoot induction from petiole explants of tembesu (Fagraea fragrans Roxb) using 6-benzylaminopurine

In vitro shoot induction from petiole explants of tembesu (Fagraea fragrans Roxb) using 6-benzylaminopurine

Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

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