The number of death couse of infection continues to increase. Treatment of infections is increasingly difficult due to increased resistance of germs to existing antimicrobial compounds. A new source of antimicrobial substances is needed. One source of antimicrobial substances is endophytic bacteria that live in the stems of Andalas plants. The aim of this study was to compare the diversity of endophytic bacteria present in old and young Andalas plant stems. The method used to isolate endophytic bacteria from the stem of the Andalas plant is the streak plate method. Bacterial identification is done macroscopically and microscopically. The results of macroscopic observation showed that there are differences in the number of isolates found in old and young Andalas plant stems. 11 isolates of endophytic bacteria from Andalas plant stems, each of which consists of 9 isolates of endophytic bacteria from old Andalas stems and two isolates from young Andalas stems. A microscopic identification was found that of the 11 isolates, eight isolates were gram-positive and three isolates were gram-negative bacteria..
Thermophilic bacteria are bacteria that can produce thermostable enzymes and used in various industries. Thermostable enzymes that can be produced by thermophilic microorganisms one of them is xylanase enzyme. Xylanase produced by microbes has optimum temperature characteristics and more diverse optimum pH on various substrates, which will affect the activity of the resulting xylanase enzyme. The influence of temperature greatly determines the activity of the enzyme at the time of catalyzing a reaction. At optimum pH conditions, the enzyme has an active side conformation that is substrate-like so that it can form a complex of appropriate enzymes and produce the product to its full potential. One of the thermophilic bacterial habitats of hot springs in West Sumatera is the Aro Sapan River hot spring located in Koto Parik Gadang Subdistrict in Ateh, South Solok District. The Saw Aro River hot spring has a temperature of 75 ° C and is pH 8 or alkaline. The aim to this research was to know the profile of the growth of thermophilic bacteria. This research is a descriptive method. The data obtained were analyzed descriptively. The conclusion is the best activity of xylanase thermophilic bacteria was at the sixth time incubation.
Halophilic bacteria is microorganism that live in high salinity environments up to 30%. The bacteria can be found in foods preserved by salting like salted fish. Salted fish is preserved foods are processed by salting and drying. The bacteria that live and ruin salted fish are halophilic bacteria and heterotoleran bacteria like halobacterium, micrococcus, bacillus, pediococcus, peseudomonas and vibrio. This observation aims to determine type of halophilic bacteria found in talang fish. This is descriptif observation held january up to february 2015 is microbiology laboratory FMIPA UNP and balai veteriner laboratory Bukittinggi. Research methods is cup statterplot methods. Sample is talang fis from Aia Bangih, west pasaman. Based on observation found isolate halophilic bacteria. Its belong to gram positve like coccus. From both, the isolates not capable produce endosopra. Talang fish from Aia Bangih, west pasaman contain two isolate halophilic bacteria consist of a genus and two species.
This study aims to determine the effect of the mindmap-assisted STEM approach on students' Higher Order Thinking Skills in learning biology class X SMA Negeri 4 Kerinci. This research is experimental research with a group pretest-posttest design model. The population in this study came from students of class X MIPA SMA Negeri 4 Kerinci in the academic year 2021/2022. The research sample was students of class X MIPA 2 and Class X MIPA 4. The sampling technique used was the random sampling technique. The data collection technique in this study was in the form of an objective test consisting of 20 HOTS questions that had been validated by experts. Data analysis is quantitative data analysis with tests using the SPSS 21 application. The results of the study can be concluded that the STEM approach has a significant effect on students' higher-order thinking skills in learning biology with the results of hypothesis testing 0.00 < 0.05. Furthermore, the STEM approach can increase students' HOTS in biology learning, the average score of the experimental class students is 85.76 and the control class is 70.31.
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