Isolation and Identification of Endophytic Bacteria from the Andalas Plant Stem (Morus macroura Miq.)

Abstract

The number of death couse of infection continues to increase. Treatment of infections is increasingly difficult due to increased resistance of germs to existing antimicrobial compounds. A new source of antimicrobial substances is needed. One source of antimicrobial substances is endophytic bacteria that live in the stems of Andalas plants. The aim of this study was to compare the diversity of endophytic bacteria present in old and young Andalas plant stems. The method used to isolate endophytic bacteria from the stem of the Andalas plant is the streak plate method. Bacterial identification is done macroscopically and microscopically. The results of macroscopic observation showed that there are differences in the number of isolates found in old and young Andalas plant stems. 11 isolates of endophytic bacteria from Andalas plant stems, each of which consists of 9 isolates of endophytic bacteria from old Andalas stems and two isolates from young Andalas stems. A microscopic identification was found that of the 11 isolates, eight isolates were gram-positive and three isolates were gram-negative bacteria..

Application of Microgranule from IAA Producing-Endophytic Bacterial as Biofetilizer on Rubber Plants (Hevea brasiliences Muell. Arg.)

“…Endophytic bacteria from one plant will produce different species from the endophytic bacteria of other plants. This is supported by the statement (Afifah et al, 2018), endophytic bacterial diversity can be influenced by nutrient availability within plant tissues. The diversity of endophytic bacteriaalso affects the secondary metabolites produced.…”

ISOLATION OF ENDOPHYTIC BACTERIA FROM TAXUS ROOT (Taxus sumatrana) AND TEST ITS POTENCY AS PRODUCER OF ANTIMICROBIAL COMPOUNDS

“…Each colony that has a different morphology is photographed for documentation. Microscopic observations were made by the Gram staining technique according to Afifah et al (2018). The stained bacteria are observed under a microscope to see the shape of the cells and the type of gram (Pelczar 2019).…”

Potensi Bakteri Endofit Asal Tumbuhan Katemas (Euphorbia heterophylla L.) sebagai Pengendali Bakteri Penyebab Penyakit Busuk Lunak (Erwinia chrysanthemi)

“…Bakteri endofit dari satu tanaman akan menghasilkan spesies yang berbeda dengan bakteri endofit tanaman lainnya. Menurut (Afifah et al, 2018), keragaman bakteri endofit dapat dipengaruhi oleh ketersediaan nutrisi di dalam jaringan tanaman. Keragaman bakteri endofit juga mempengaruhi metabolit sekunder yang dihasilkan.…”