This paper investigates the effect of different concentrations of nanostructured materials: fullerene‐like (C60), onion‐like carbon (OLC) and ultradispersed diamonds (UDD) on the formation of multicellular spheroids. The chemical composition and purity of nanomaterials are controlled by Fourier transform infrared spectroscopy. The strength and direction of the impact of nanomaterials on the cell population was assessed using microphotography of tumor multicellular spheroids of breast adenocarcinoma cell line MCF‐7 in vitro and Pearson's correlation coefficient. The results demonstrated that UDD and OLC reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids of ∼3 × 10−3 mm3 in significant amount. The fullerenes reduced in the main cell adhesion to substrate that led to formation of cell aggregates of ∼5 × 10−3 mm3. The results could be useful for achievement of the directed cell growth in three‐dimensional culture.
Structural changes occurring in C 60 fullerenes after ball-milling processing were studied. The quantitative characteristics of the local atomic configurations were reconstructed by the reverse Monte Carlo method in the structure of pristine and ball-milled fullerenes C 60 , respectively. It is shown that 3-fold atomic rings, indicating complete amorphization of crystalline fullerenes, are dominant in the structure of material after 14 h treatment. The behavior of the distribution of the sphericity coefficient of Voronoi polyhedra validates a gradual structural disordering of molecular crystal of C 60 and, respectively, the formation of amorphous carbon with randomly arranged closepacked structure after continuous ball-milling treatment.
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