Hot springs are a common source of thermophyles which produce thermostable enzymes. The objective of this study was to isolate and identify thermostable amylase-producing bacteria from a local geothermal spring. An amylase-producing bacterium strain was isolated from this hot spring which excreted amylase after being grown on starch agar screening plates at 37 °C. It was identified as Bacillus megaterium using the method of 16S ribosomal DNA. The organism is a rod-shape and is a spore-forming bacterium. Maximum amylase production was achieved after incubation in the production media for 72 h. Preliminary analysis of the secreted amylase showed that the enzyme could bind to DEAE-Sepharose matrix and was discharged by eluting with 0.5 M NaCl. The partially purified enzyme was stable up to 75 °C, showing that this enzyme might have potential application in the starch-processing industry.
Bacillus, hotsprings, isolation, thermostable amylaseSumber air panas merupakan habitat umum mikroba termofilik yang menghasilkan enzim-enzim termostabil. Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi bakteri penghasil amilase termostabil dari sumber mata air panas lokal. Satu strain bakteri penghasil enzim amilase berhasil diisolasi. Bakteri tersebut menghasilkan enzim amilase setelah ditumbuhkan pada suhu 37 °C pada media agar yang mengandung pati. Hasil analisis gen subunit 16S rRNA menunjukkan bahwa bakteri tersebut adalah Bacillus megaterium. Bakteri tersebut berbentuk batang dan menghasilkan spora. Produksi enzim amilase maksimum diperoleh setelah bakteri dikultivasi pada media produksi selama 72 jam. Analisis pendahuluan enzim yang disekresi menunjukkan bahwa enzim tersebut terikat pada matriks DEAE-Sepharose dan terlepas dari matriks setelah dielusi dengan larutan NaCl 0,5 M. Amilase dari fraksi NaCl 0,5 M tetap aktif hingga suhu 75 °C. Hal ini menunjukkan bahwa enzim amilase ini mungkin memiliki potensi untuk digunakan pada industri pengolahan tepung pati. amilase termostabil, Bacillus, isolasi, air panas
Tujuan dilakukannya penelitian ini yaitu untuk mengetahui dampak larvasida dari daun lidah buaya (Aloe vera) terhadap mortalitas nyamuk (Culex quinquefasciatus say). Sampel yang digunakan dalam penelitian merupakan larva nyamuk Culex quinquefasciatus say sebanyak 625 ekor yang didapatkan dari Laboratorium Entomologi Tropical Disease Diagnostik Center (TDDC), kampus C UNAIR. Kelompok perlakuan terdiri dari P01 sebagai kontrol negatif larva nyamuk diberikan aquades, kelompok P02 kontrol positif larva nyamuk diberi perlakuan dengan bubuk abate, kelompok P1 larva nyamuk diberi perlakuan ekstrak daun lidah buaya 10%, kelompok P2 larva nyamuk diberi perlakuan 15% ekstrak daun lidah buaya dan kelompok P3 larva nyamuk diberi perlakuan ekstrak daun lidah buaya 20%, pengamatan mortalitas larva dilakukan setiap 2 jam dalam dua belas jam pada waktu satu hari. Data mortalitas larva nyamuk yang didapatkan dianalisis dengan spss ANOVA dan dilanjutkan dengan uji Duncan. Hasil yang didapatkan pada jam ke 6 merupakan hasil yang sangat efektif terhadap tingkat mortalitas dengan nilai rata-rata pada kelompok perlakuan 1 sebesar 16 ± 1.58, kelompok perlakuan 2 sebesar 20.80 ± 0.83, kelompok perlakuan 3 sebesar 23 ± 0.70.
We present population structure analysis of two differed Ongole grade cattle strains in Indonesia i.e., common Ongole grade (PO) and Kebumen Ongole grade (PO Kebumen). The aim of this study was to identify the genomic characteristic of both PO and PO Kebumen cattle strains. In this study, genotyping method of 15 PO and 25 PO Kebumen was carried out using an Illumina BovineSNP50 Bead Chip. In order to deeper analyses, we used additional 50K SNP data of 20 PO, 20 Nellore, 20 Brahman, 20 Simmental, and 20 Limousin that freely available in the data repositories. An estimation of the genetic population study was carried out using following approaches: pairwise fixation indices, heterozygosity and Nei’s standard genetic distance, and ancestral based admixture. These analyses revealed a B. taurus influence in PO but none in PO Kebumen. Furthermore, we also identified the close admixture of PO Kebumen to Nellore. Since Nellore was originated from Ongole cattle, we presume the PO Kebumen is the maintained Pure Ongole Breed in Indonesia.
The purpose of this study was to evaluate the potencial influence local product Abpo PMSG treatment on mice foetus number. Methode used for this research was by injecting 0,1 ml Abpo PMSG on superovulated mice with PMSG and hCG. There were six treatment in this research. Control group was group of mice that only superovulated. P1 was given PMSG and Abpo PMSG together. P2 was group of mice that wich Abpo given 1 hour after PMSG, P3 given Abpo PMSG when they were estrous. P4 given Abpo PMSG 1 hour before hCG, and P5 given Abpo PMSG 1 hour after hCG. The result showed that the most foetus number resulted from P3. The least foetus number was from control group. Foetus number of P1, P2, P4 and P5 was showed no significant difference with P3 group ( P > 0,05).Keywords: PMSG, Abpo PMSG, foetus number
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