Hot springs are a common source of thermophyles which produce thermostable enzymes. The objective of this study was to isolate and identify thermostable amylase-producing bacteria from a local geothermal spring. An amylase-producing bacterium strain was isolated from this hot spring which excreted amylase after being grown on starch agar screening plates at 37 °C. It was identified as Bacillus megaterium using the method of 16S ribosomal DNA. The organism is a rod-shape and is a spore-forming bacterium. Maximum amylase production was achieved after incubation in the production media for 72 h. Preliminary analysis of the secreted amylase showed that the enzyme could bind to DEAE-Sepharose matrix and was discharged by eluting with 0.5 M NaCl. The partially purified enzyme was stable up to 75 °C, showing that this enzyme might have potential application in the starch-processing industry. Bacillus, hotsprings, isolation, thermostable amylaseSumber air panas merupakan habitat umum mikroba termofilik yang menghasilkan enzim-enzim termostabil. Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi bakteri penghasil amilase termostabil dari sumber mata air panas lokal. Satu strain bakteri penghasil enzim amilase berhasil diisolasi. Bakteri tersebut menghasilkan enzim amilase setelah ditumbuhkan pada suhu 37 °C pada media agar yang mengandung pati. Hasil analisis gen subunit 16S rRNA menunjukkan bahwa bakteri tersebut adalah Bacillus megaterium. Bakteri tersebut berbentuk batang dan menghasilkan spora. Produksi enzim amilase maksimum diperoleh setelah bakteri dikultivasi pada media produksi selama 72 jam. Analisis pendahuluan enzim yang disekresi menunjukkan bahwa enzim tersebut terikat pada matriks DEAE-Sepharose dan terlepas dari matriks setelah dielusi dengan larutan NaCl 0,5 M. Amilase dari fraksi NaCl 0,5 M tetap aktif hingga suhu 75 °C. Hal ini menunjukkan bahwa enzim amilase ini mungkin memiliki potensi untuk digunakan pada industri pengolahan tepung pati. amilase termostabil, Bacillus, isolasi, air panas
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