At 24h post-HI, lesion size on DWI, using 80% ADC threshold is the best predictor of final infarct volume. Although T2WI performed less well, it has the advantage of superior spatial resolution and is technically less demanding. These are important considerations for experiments which utilize MRI as a surrogate method for lesion quantification in the neonatal rat HI model.

Perinatal hypoxia and ischemia (HI) are a significant cause of mortality and morbidity. To understand the molecular mechanisms for HI-induced brain damage, here we used a proteomic approach to analyze the alteration and modification of proteins in neonatal mouse brain 24 h after HI treatment. Significant changes of collapsin response mediator proteins (CRMPs) were observed in HI brain. CRMPs are a family of cytosolic proteins involved in axonal guidance and neuronal outgrowth. We found that CRMP2, CRMP4 and CRMP5 proteins were altered post-translationally after HI treatment. Mass spectrometric and Western blot analyses detected hypophosphorylated CRMP proteins after HI. Further analysis of CRMP kinases indicated inactivation of cyclin dependent kinase 5 (CDK5), a priming kinase of CRMPs and a neuronal specific kinase that plays pivotal roles in neuronal development and survival. The reduction of CDK5 activity was associated with underexpression of its activator p35. Taken together, our findings reveal HI-induced dephosphorylation of CRMPs in neonatal brain and suggest a novel mechanism for this modification. Hypophosphorylated CRMPs might be implicated in the pathogenesis of HI-related neurological disorders.

Background: Left ventricular assist device (LVAD) has been increasingly used in patients with advanced heart failure. This study aimed to assess the impact of implementation of LVAD therapy on heart transplantation (HTx) service in Hong Kong (HK). Methods: LVAD program was started in 2010 in HK and patients who had been put on HTx waiting list since the start of HTx program in HK from 1992 to 2020 were included for analysis. Survival on HTx waiting list between pre-LVAD era 1992-2009 and post-LVAD era 2010-2020 were analyzed by Kaplan-Meier method and compared by log-rank test. Multivariate analysis by time-dependent Cox-proportional hazard model was used to identify independent predictors of HTx waiting list mortality. Results: A total of 478 heart transplant listing episodes involving 457 patients were included for analysis.There were 232 heart transplantations (HTxs), including one re-transplantation, during the study period.There were 110 patients who received LVAD as bridge to transplantation (BTT) and 30 of them had undergone subsequent HTx. The 1-, 2-and 3-year survival on waiting list were 82.3%, 61.7% and 43.0% respectively in the pre-LVAD era (n=178), while the 1-, 2-and 3-year survival were significantly improved at 85.7%, 81.8% and 78% respectively in the post-LVAD era (n=300), (P=0.003). Time-dependent multivariate analysis revealed that LVAD support was independently associated with significant reduction of waiting list mortality [odds ratio (OR): 0.21; 95% confidence interval (CI): 0.10-0.44, P<0.001]. There was no significant difference when comparing survival after LVAD as BTT and survival after HTx up to 8 years (76.1% vs. 72% at 8 years respectively, P=0.732). Conclusions: Waiting list survival improved in the post-LVAD era driven by the implementation of LVAD service. Long-term survival for LVAD recipients as BTT were comparable to heart transplant recipients in HK.

Abstract. Background: Child abuse and suicide among the young population is a serious and prevalent problem. Many studies have demonstrated that people with adverse childhood experiences, such as child abuse, are likely to develop suicidal behavior. This study evaluates the connection between child abuse and suicidal behavior in the Hong Kong community where incidents of child abuse have been on the rise over the past decade. Aims: To determine the association between child abuse and attempted suicide in the child population of Hong Kong using hospital electronic medical records system. Method: From January 1, 1995 to July 31, 2016, patients with admission age < 18 years with the diagnosis of child abuse or influenza infection (comparison group) were included in this study ( n = 54,256). In secondary data analysis, an association was found between children who had experienced child abuse and the outcome measure of hospital admission for attempted suicide compared with influenza infection. Results: The adjusted hazard ratio of attempting suicide in children who experienced sexual abuse and physical abuse compared with the influenza-infected group was 6.48 (95% CI [4.56, 9.19]) and 4.83 (95% CI [3.67, 6.34]). The age at onset of adverse incidents was negatively associated with the attempted suicide timing. Female patients had a 1.64 higher risk of repeating attempted suicide. In addition, nearly 5% of children who had experienced child abuse attempted suicide in the 10 years after their admission, and more than 36% of patients had a record of repeated suicide attempt in the 20 years after the initial admission. Limitations: The accuracy of the diagnosis, selection bias, insufficiency of study period, Berkson's bias, incomplete socioeconomic status, as well as the absence of psychiatric diagnosis are the limitations. Conclusion: Our results indicate that there is a significant association between child abuse and suicide attempts in Hong Kong. If confirmed, the study (a) demonstrates that hospital admission records are a critical source for identifying children with a high risk for suicidal behavior; (b) may inform policy makers that additional and long-term intervention programs should be provided to children so as to reduce subsequent suicide attempts.

Background: Stroke remains one of the most important complications of cardiac surgery and occurs in 2.2% after open-heart procedure. It is associated with significant morbidity and mortality. The use of a cerebral protection system during transcatheter aortic valve implantation may be associated with a lower risk of periprocedural strokes, and mortality at 30 days. The aim of the present study was to assess the safety and feasibility of this device in patients at high risk for stroke during open cardiac surgery. Methods: We present six patients with a high risk of perioperative stroke who underwent placement of Sentinel cerebral protection system during various openheart operations between 2018 and 2021. Results:The system was successfully deployed, and debris was retrieved in all patients. There was no device-related complication or development of ischemic stroke postoperatively. One patient suffered from intracranial hemorrhage due to peri-operative coagulopathy.Conclusions: We demonstrated the feasibility and safety of this hybrid approach with a high debris capture rate. It encourages further study to evaluate the benefits of the Sentinel cerebral protection system in reducing stroke and mortality in selected patients undergoing open-heart surgery.