AbstrakMethicillin resistant Staphylococcus aureus (MRSA) merupakan salah satu galur multiresisten yang menjadi masalah kesehatan global sejak 50 tahun terakhir. Secara genetik, resistensi MRSA didasari adanya insersi mecDNA atau Staphylococcal cassette chromosome mec (SCCmec) pada kromosom S. aureus. Sejauh ini telah diidentifikasi 5 tipe SCCmec, yaitu SCCmec tipe I-V yang berasal dari isolat di seluruh dunia. Akhir-akhir ini ditemukan perubahan pola penyebaran, pola kepekaan terhadap antimikrob, dan perubahan kandungan SCCmec. Oleh karena itu identifikasi SCCmec menjadi demikian penting untuk mengetahui adanya perubahan tersebut. Tujuan penelitian ini adalah untuk mengidentifikasi tipe SCCmec dari isolat MRSA yang diperoleh di rumah sakit.
AbstrakMetil selenosistein (MSC) merupakan bentuk selenium yang paling efektif melawan sel kanker. Pembentukan MSC diperantarai oleh enzim seleno metiltransferase (disandi gen smt) sebagai mekanisme detoksifikasi selenium dengan cara metilasi selenosistein. Gen smt telah dikarakterisasi dari tumbuhan yang kaya selenium, Astragalus bisulcatus.
Staphylococcus aureus (S.aureus) remains to be the most important cause of post operative wound infection. Nurses could become reservoirs to transmit S.aureus through contaminated hands transiently, or through colonized nose. Strain polymorphism could be determined by Restriction Fragment Length Polymorphism (RFLP), using coa gene and restriction endonuclease enzyme Alu1. There were 30 isolates of S.aureus's infection, and 20 isolates taken from hands and nose of the nurses in charge. From 50 isolate positive S.aureus, PCR results showed single and multiple bands within 300 to 900 base pairs (bp) in length, and multiple bands within 200 to 600 bp. Five out of 30 patients (17%) showed no PCR-RFLP similarity with any of the nurses. Ten out of 15 nurses which hands were positive for S.aureus, has PCR-RFLP similarity with some patients. There was only 1 out of 5 nurses which nose was positive for S.aureus, showed PCR-RFLP similarity with some patients. Statistically, the proportion of the similar PCR-RFLP between those samples in this study is 0.12 (12%). Conclusion: Nurses had 12 % PCR-RFLP similarity for S.aureus with post operative wound infection.
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