Identifikasi Staphylococcal Cassette Chromosome Mec Methicillin Resistant Staphylococcus aureus dengan Polymerase Chain Reaction

Yuwono,; Sa, Sunarjati; Masria, Sadeli; Supardi, Imam

doi:10.15395/mkb.v43n2.52

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Berdasarkan penelitian Yuwono (2023) pada kopi dan teh ditemukan senyawa H2O2 yang terbukti memiliki efek antibakteri. Mekanisme H2O2 membunuh bakteri dengan cara menghasilkan radikal hidroksil yang dapat merusak lipid membrane, DNA, dan komponen sel lainnya (21).…”

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Perbandingan Efektivitas Antibakteri Ekstrak Air Kopi Arabika dengan Ekstrak Air Teh Hitam terhadap Kultur Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) ATCC© 33591™ Secara Eksperimental in Vitro

Rizky Alifian Ramadhan,

Hendro Sudjono Yuwono,

Meike Rachmawati

2024

BCSMS

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Abstract. S. aureus infections often occur in a community or hospital environment and management is still difficult due to the emergence of strains that are resistant to many antibiotics such as Methicillin-resistant Staphylococcus aureus (MRSA). Herbal medicine is an alternative treatment that is often used, including Arabica Coffee (Coffea Arabica) and black tea (Camelia Sinensis). Arabica coffee and black Tea contain polyphenols known as caffeine, chlorogenic acid and catechins. The catechins in black tea, namely epigallocatechin gallate (EGCG) and chlorogenic acid in coffee, have been proven to have antibacterial power against gram-positive bacteria. In gram-positive bacteria, EGCG and chlorogenic acid are able to inhibit the synthesis of the bacterial wall. This research used an experimental in vitro study with the method used by Kirby Bauer disc diffusion with 5 samples, Arabica coffee water extract and black tea water extract in two concentrations, 50% and 100% as treatment, and the antibiotic Vancomycin with a concentration of 100% as a positive control. The research results showed that Arabica coffee water extract had an average zone of inhibition, at a concentration of 100% (14.2 mm) and a concentration of 50% (9.7 mm) with a probability value (p-value) of 0.009 (p≤0, 01), while an inhibition zone was found in black tea water extract at a concentration of 100% (16.2 mm) and a concentration of 50% (11.5 mm) with a probability value (p-value) of 0.009 (p≤0.01) against Methicillin-resistant S. aureus. In conclusion, the inhibitory power of Black Tea water extract is stronger than that of Arabica coffee water extract against Methicillin-resistant Staphylococcus aureus bacteria. Abstrak. Infeksi S. aureus sering terjadi pada suatu komunitas atau lingkungan rumah sakit dan untuk pengelolaannya menjadi sulit karena munculnya strain yang resisten terhadap banyak obat antibiotik seperti Methicillin-resistant Staphylococcus aureus (MRSA). Obat herbal merupakan salah satu pengobatan alternatif yang sering dipergunakan, diantaranya terdapat kopi Arabika (Coffea Arabika) dan teh hitam (Camelia Sinensis). Kopi arabika dan teh hitam memiliki kandungan polifenol yang dikenal sebagai kafein, asam klorogenat dan katekin. Katekin pada teh hitam yaitu epigallocatechin gallat (EGCG) dan asam klorogenat pada kopi terbukti memiliki daya antibakteri terhadap bakteri gram positif. Pada bakteri gram positif EGCG dan asam klorogenat mampu menghambat sintesis dari dinding bakteri. Penelitian ini menggunakan studi eksperimental in vitro dengan metode yang digunakan disc diffusion Kirby Bauer dengan 5 sampel, yakni ekstrak air kopi Arabika dan ekstrak air teh hitam dalam dua konsentrasi yaitu 50% dan 100% sebagai perlakuan, dan antibiotik Vancomycin degan konsentrasi 100% sebagai kontrol positif. Hasil penelitian didapatkan ekstrak air kopi Arabika memiliki rata-rata zona inhibisi yaitu pada konsentrasi 100%, (14,2 mm) dan konsentrasi 50% (9,7 mm) dengan nilai probabilitas (p-value) sebesar 0,009 (p≤0,01), sedangkan ditemukan zona inhibisi pada ekstrak air teh hitam pada konsentrasi 100%, (16,2 mm) dan konsentrasi 50% (11,5 mm) dengan nilai probabilitas (p-value) sebesar 0,009 (p≤0,01) terhadap Methicillin-resistant S. aureus. Kesimpulannya, bahwa daya hambat ekstrak air Teh Hitam lebih kuat dibandingkan dengan ekstrak air kopi Arabika terhadap bakteri Methicillin-resistant Staphylococcus aureus.

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Section: Aunclassified

Perbandingan Efektivitas Antibakteri Ekstrak Air Kopi Arabika dengan Ekstrak Air Teh Hitam terhadap Kultur Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) ATCC© 33591™ Secara Eksperimental in Vitro

Rizky Alifian Ramadhan,

Hendro Sudjono Yuwono,

Meike Rachmawati

2024

BCSMS

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Bioprospecting of Bacterial Endophytes fromCurcuma aeruginosa, Curcuma xanthorrhizaandCurcuma zedoariaas Antibacterial Against Pathogenic Bacteria

Indrawati

Rossiana

Diresna

2018

IOP Conf. Ser.: Earth Environ. Sci.

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IDENTIFICATION OF SCCMEC TYPE IN ISOLATE OF METHICILLIN RESISTANT Staphylococcus aureus (MRSA) IN JAMBI BY USING POLYMERASE CHAIN REACTION (PCR)

humaryanto

hanina²,

Lipinwati³

et al. 2020

IJTID

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Staphylococcal cassette chromosome mec (SCCmec) is one of the mobile genetic elements of Methicillin-Resistant Staphylococcus aureus (MRSA) that carries many resistance genes and allows SCCmec to move from one bacterium to another. Twelve types of SCCmec have been identified throughout the world. Identification of SCCmec type is needed to determine the pattern of MRSA resistance in a particular region. This study aimed to identify the type of SCCmec MRSA from clinical samples. Specifically, this study was conducted at the Biomolecular Laboratory of the Faculty of Medicine and Health Sciences of Jambi University in June 2018-February 2019. Culture was carried out on 100 clinical specimens of festering wound swabs from inpatients at hopitals in Jambi City. A total of 32 samples of Staphytect plus test positive were tested using Cefoxitin disc diffusion method and MecA Polymerase Chain Reaction (PCR). There were 14 samples identified as MRSA isolates, namely twelve samples (85.72%) of SCCmec type III, one sample (7.14%) of SCCmec type II, and one sample (7.14%) of SCCmec type IVb. The results were different from previous studies where all MRSA isolates (100%) in Indonesia were SCCmec type III, although most SCCmec types were still dominated by SCCmec type III. This study concludes that there has been a shift in the content of SCCmec in MRSA isolate originating from hospitals in Jambi city.

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Identifikasi Staphylococcal Cassette Chromosome Mec Methicillin Resistant Staphylococcus aureus dengan Polymerase Chain Reaction

Cited by 3 publications

References 19 publications

Perbandingan Efektivitas Antibakteri Ekstrak Air Kopi Arabika dengan Ekstrak Air Teh Hitam terhadap Kultur Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) ATCC© 33591™ Secara Eksperimental in Vitro

Perbandingan Efektivitas Antibakteri Ekstrak Air Kopi Arabika dengan Ekstrak Air Teh Hitam terhadap Kultur Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) ATCC© 33591™ Secara Eksperimental in Vitro

Bioprospecting of Bacterial Endophytes fromCurcuma aeruginosa, Curcuma xanthorrhizaandCurcuma zedoariaas Antibacterial Against Pathogenic Bacteria

IDENTIFICATION OF SCCMEC TYPE IN ISOLATE OF METHICILLIN RESISTANT Staphylococcus aureus (MRSA) IN JAMBI BY USING POLYMERASE CHAIN REACTION (PCR)

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