Biosurfaktan merupakan zat penurun tegangan permukaan yang dihasilkan oleh mikroorganisme yang memiliki aktivitas antibakteri. Bacillus cereus merupakan salah satu bakteri penghasil biosurfaktan. Penelitian ini bertujuan untuk mendapatkan kondisi optimum produksi biosurfaktan dari Bacillus cereus dengan memanfaatkan minyak jelantah sebagai sumber karbon. Optimasi produksi biosurfaktan dilakukan pada variasi konsentrasi minyak jelantah 2%, 3%, 4%, 5% dan variasi pH media 6, 7, 8 serta dilakukan uji aktivitas menggunakan metode indeks emulsifikasi 24 jam (IE24). Penelitian ini menggunakan media produksi Mineral Salt Medium (MSM), konsentrasi inokulum sebanyak 10% (108 CFU/ml), inkubasi pada suhu ruang dengan kecepatan agitasi 160 rpm. Ekstraksi biosurfaktan menggunakan pelarut kloroform : metanol (2:1). Konsentrasi minyak jelantah terbaik adalah 3% dan nilai pH terbaik pada pH 6. Hasil produksi biosurfaktan dari bakteri Bacillus cereus adalah 8,9 g/L dengan nilai IE24 57,17%. Hasil dari penelitian ini diharapkan mendapatkan kondisi optimum untuk produksi biosurfaktan yang mengandung antimikroba yang dihasilkan oleh Bacillus cereus.
Biosurfactants are surface tension lowering agents produced by microorganisms which have advantages in many respects over synthetic surfactants. Biosurfactants are more environmentally friendly and can be used widely in pharmaceutical field. One of the bacteria that has not been widely explored as biosurfactants producer is Brevudimonas terrae. The production of biosurfactants in these bacteria also needs to be optimized, including by using waste frying oil as a carbon source. The purpose of this study was to obtain optimum conditions in the production of bio-surfactants by utilizing waste frying oil as a carbon source from this bacteria. In this study, variations in the optimized production conditions included the concentration of waste frying oil, labelled 2%, 3%, 4%, and 5% and the medium pH at 6, 7, and 8. The study was using Mineral Salt Medium as production medium, the amount of inoculum concentration was 10% v/v, agitation speed 160 rpm and incubation at room temperature. The optimum conditions for biosurfactant production were determined based on the best emulsification index. The biosurfactant extraction was carried out using a combination of chloroform and methanol (2:1) solvents. The best concentrations of waste frying oil for Brevundimonas terrae was 3%, and the best medium pH was 7. Biosurfactants produced from this bacteria amounted to 9,63 g/L with an emulsification index 61,25%.
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