Candida auris is an emergent yeast pathogen, easily transmissible between patients and with high percent of multidrug resistant strains. Here we present a draft genome sequence of the first known Russian strain of C. auris, isolated from a case of candidemia. The strain clustered within South Asian C. auris clade and seemingly represented an independent event of dissemination from the original species range. Observed fluconazole resistance was probably due to F105L and K143R mutations in ERG11.
Background: Patients with haematological malignancies often require central venous access for administration of chemotherapy and blood products. Infection remains one of the commonest complications of these devices and is associated with significant morbidity and sometimes mortality. Aims:The purpose of our study is to identify clinical and laboratory factors which may predict a higher risk of line-associated bloodstream infections in patients with haematological malignancies. Methods: Patients treated for haematological malignancies at our centre between January 2016 and December 2018 were retrospectively identified. Only patients who had either a tunnelled line or peripherally-inserted central catheter (PICC) were included in the study. Line-associated bloodstream infections were recorded and the predictive value of a number of baseline clinical and laboratory parameters for infections was assessed. Results: One hundred and fifty-three patients were identified during the study period. The haematological diagnosis was as follows: acute leukaemia in 63 (41.2%) patients, lymphoma in 81 (52.9%) and other diagnoses in nine (5.9%). One hundred and twenty-five patients (81.7%) had a tunnelled line while 28 patients (18.3%) had a PICC. The total duration of follow-up was 10,416 line-days with a median of 55 days. The median in-situ duration for tunnelled lines was 38 days (range 4-150 days) while that for PICCs was 17 days (range 2-145 days) (P = 0.02). Bloodstream infections were identified in 58 (37.9%) patients, with 28 patients (18.3%) satisfying the criteria for a catheter-related bloodstream infection (CRBSI). The overall infection rate was 5.5 infections per 1,000 line-days. There was no significant difference in the rates of infection between tunnelled lines and PICC lines (P = 0.25). On multivariate analysis for the whole group, line-associated infections were more likely in patients with acute leukaemia (P < 0.001), those receiving corticosteroids as part of the chemotherapy regimen (P = 0.01) and those with neutropenia (absolute neutrophil count <1.0 x 10 9 /L) at the time of line placement (P = 0.03). On univariate analysis, predictive factors for line infections in tunnelled catheters included acute leukaemia (P < 0.001), steroid use (P = 0.01), younger age (P = 0.04) and total number of line-days with neutropenia (P = 0.04). On multivariate analysis only acute leukaemia (P < 0.001) and steroid use (P = 0.06) retained independent prognostic significance. For PICCs only neutropenia at the time of placement was predictive for an increased risk of infection (P = 0.01). Summary/Conclusion:In conclusion this study identified acute leukaemia, corticosteroid use and neutropenia at the time of catheter placement as clinical predictors of infection in patients with haematological malignancy.
The article provides a method of isolating a lipase producer and identification methods by classical methods in microbiology of an isolated micromycete producing lipase. The species affiliation of Penicillium hordei has been determined. The temperature of micromycete cultivation was studied, as well as the dynamics of Penicillium hordei lipase biosynthesis. Screening plans were also carried out (Plackett-Berman Plan), then optimization according to the steep climb plan and an experiment based on the plan of a full-factor experiment (three-level CFE) was also conducted to determine the optimal concentrations that are significant for lipase biosynthesis of components. A mathematical model is constructed that describes the relationship between the influence of two significant factors on the volumetric activity of P. Hordei culture fluid. The most optimal composition for the culture fluid for lipase biosynthesis, Penicillium hordei micromycete, was compiled.
Научно-исследовательский институт медицинской микологии им. П.Н. Кашкина Федерального государственного бюджетного образовательного учреждения высшего образования «Северо-Западный государственный медицинский университет им. И.И. Мечникова» Министерства здравоохранения Российской Федерации, 194291, г. Санкт-Петербург, Российская Федерация 2 Федеральное государственное бюджетное образовательное учреждение высшего образования «Северо-Западный государственный медицинский университет им. И.И. Мечникова» Министерства здравоохранения Российской Федерации, 191015, г. Санкт-Петербург, Российская Федерация 3 Санкт-Петербургское государственное бюджетное учреждение здравоохранения «Городская больница № 40 Курортного района», 197706, г. Санкт-Петербург, Российская Федерация 4 Национальное агентство клинической фармакологии и фармации, 115088, г. Москва, Российская Федерация
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