This research was conducted at the plant tissue culture lab. - College of Agriculture – University of Baghdad from February 2015 to May 2016. The Study was aimed to investigate the in vitro propagation of Moringa oleifera, by inoculation a single nodal segment in MS medium supplemented with different concentrations of plant growth regulators. The best responded of single node reached to 90% was achieved with MS medium supplemented with 1 mg. L-1 BA and 0.2 mg. L-1 IAA. At Multiplication stage results showed that MS medium supplemented with 2 mg. L-1 of BA with 0.1 mg. L-1 IAA increased numbers of shoot comparing with the Kin; (6.40 shoot /exp.) While the treatment of MS medium supplemented with 0.2 mg. L-1 IAA without BA gave the best shoots length which reached 4.15 cm. In rooting stage, shoots have been cultured in MS medium supplemented with different concentrations of Auxins. Results showed that MS medium at half strength supplemented with 1 mg. L-1 of IBA and 1.5 mg. L-1 IAA significantly increased the number of roots per shoot, roots length up to (7.2 roots/shoot and 6.14 cm) respectively. The survival percentage of plantlets was 70% when they planted in a composed consisted of 3:1 (v:v) peatmoss: soil mixture. We found that BA increased numbers of shoot comparing with the Kin and MS medium at half strength was the best in rooting plantlet comparing with MS medium at full strength.
An experiment was conducted to study the effect of sucrose, poly ethylene glycol (PEG) on hypocotyl induced callus of Moringa oleifera at the plant tissue culture lab.- College of Agriculture– University of Baghdad from February 2015 to May 2016. Sucrose concentrations were 30, 60, and 90, 120 g .L -1 and PEG 0, 25, 50 and 100 g .L -1 added to MS medium supplemented with 2.0 mg .L -1 of 2, 4-D and 0.1 mg .L -1 of NAA. MS medium supplemented with120 g .L -1 of sucrose gave the best amount of Zeatin, Quercetin and Kaempferol reached to 103.4, 1324.6 and 966.5 µg. g dry weight of callus-1 respectively. The concentrations of active compound increasing with adding PEG, MS medium supplemented with 100 g .L -1 PEG gave the highest value of Zeatin, Quercetin and Kaempferol which recorded 92.01, 3528.0 and 931.0 µg. g dry weight of callus-1 respectively. We found that we could increase the production of active material from callus that induced from explant by exposure the callus to several stress and then could separate the pure active material and used it as a drug in medicine.
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