Influence of Stress on Secondary Metabolites Production From Callus of Moringa Oleifera in Vitro

Ameen, Ibrahim

doi:10.36103/ijas.v48i4.367

Cited by 2 publications

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“…High sucrose concentration considered source of osmotic stress which used widely in tissues culture for increase active compounds Elkahoui et al( 2013. The important of adding sucrose to MS medium come from photosynthesis of plant tissues depending on addition sucrose, but in high concentrations of sucrose resulting stress increasing and low in product in primary metabolites compounds then affecting the secondary metabolites (Ameen and Mohammed ,2010).…”

Section: Effect Of Sucrose Treatments On Vinblastine and Vincristine ...mentioning

confidence: 99%

Vinblastine and Vincristine Alkaloids Production From Callus of Catharanthus roseus (L.) G. Don under Some abiotic factors

T .Khashan,

A.H.Al-Athary

2016

Kufa Jour. Bio.

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This experiment was conducted in Faculty of Science labs, Kufa University, carried out during 2013-2014 to study many experiments to induce callus tissues from leaves of (Catharanthus roseus ( L. ) G. Don in vitro using MS medium supplemented with Dichlorophenoxy actic acid (2,4-D) at difference concentrations (0.5, 1, 1.5) mg/l with the interaction of (BA) benzyl adenine at concentrations of (0.5, 1, 1.5) mg/l. Identically callus fresh weight, then use it in the next experiments and investigate the effect of abiotic factors (drought and sucrose) on vinblastine and vincristine production from callus tissue, and leaves of intact plant. This study include determination the catalase activity, proline and total soluble carbohydrates content as responsible to the variety of the stresses in callus as well as quantities and qualities determination of vinblastine and vincristine using TLC (Thin layer chromatography ) and HPLC(high performance liquid chromatography). Results reveled that the highest fresh weight of callus (3.276) g was for (1mg/l 2,4-D + 1mg/l BA ) combination treatment. However, the results showed that the additions of abiotic factors to the callus cultural medium cause difference results in fresh and dry weights of callus in accumulation phase, reducing the fresh and dry weight in all treatments expect sucrose 40 g/l treatment which gave increase in fresh and dry weight. Also, this results showed that all treatments increase total soluble carbohydrates, proline content and catalase enzyme activity with significant difference compared with control treatment and significant increase of alkaloids production with the superiority of (vinblastine and vincristine) content in Callus with abiotic factors stresses than the content in control treatment( without any factor).

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Section: Effect Of Sucrose Treatments On Vinblastine and Vincristine ...mentioning

confidence: 99%

Vinblastine and Vincristine Alkaloids Production From Callus of Catharanthus roseus (L.) G. Don under Some abiotic factors

T .Khashan,

A.H.Al-Athary

2016

Kufa Jour. Bio.

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“…For most plants, a change in one element can affect SMs content [4,6]. Many researchers investigate the accumulation of secondary products by exposing callus to different stresses [7,8].…”

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confidence: 99%

In Vitro Effect of Mannitol Stress and γ-ray on Secondary Products From Golden Sunrise Cherry Tomato Callus

Mizil

El-Kaaby

Al-Shammary

2023

IHJPAS

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Effect of drought stress induced by mannitol on secondary products in callus of Golden Sunrise cherry tomatoes were studied in vitro. Seeds were irradiated with gamma at the doses (0, 20, or 40 Gy), and germinated on Murashige and Skoog, (MS) basal medium. Callus were initiated from cotyledon leaf explants using a combination of (2.0 kin + 2.0 IAA mg. l-1) then transferred into MS medium supplemented with mannitol at concentrations of (0, 40, and 60 g.l-1). Vitamin C(vit C), enzyme activity, Ascorbate peroxidase (Apx), and Amylase enzymes, Proline amino acid and β- carotene were quantified by high-performance Performance Liquid Chromatography (HPLC). Results revealed that, highest contents of (Apx) 128.16 µg. ml-1 and 132.6 µg. ml-1 for amylase at 60 g.l-1 of mannitol and 0 Gy of gamma, respectively. Furthermore, the content of VIT C (265.57 µg. ml-1 ) at 20 Gy and 60 g.l-1 of mannitol. The results also showed that a combination of 40 Gy and 60 g.l-1 of mannitol was optimum for the production of high proline amino acids (761.0 µg. ml-1 ) and (113.54 µg. ml-1 ) for β- carotene content in callus culture.

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Influence of Stress on Secondary Metabolites Production From Callus of Moringa Oleifera in Vitro

Cited by 2 publications

References 0 publications

Vinblastine and Vincristine Alkaloids Production From Callus of Catharanthus roseus (L.) G. Don under Some abiotic factors

Vinblastine and Vincristine Alkaloids Production From Callus of Catharanthus roseus (L.) G. Don under Some abiotic factors

In Vitro Effect of Mannitol Stress and γ-ray on Secondary Products From Golden Sunrise Cherry Tomato Callus

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