In Vitro Propagation of Moringa Oleifera

Abstract

This research was conducted at the plant tissue culture lab. - College of Agriculture – University of Baghdad from February 2015 to May 2016. The Study was aimed to investigate the in vitro propagation of Moringa oleifera, by inoculation a single nodal segment in MS medium supplemented with different concentrations of plant growth regulators. The best responded of single node reached to 90% was achieved with MS medium supplemented with 1 mg. L-1 BA and 0.2 mg. L-1 IAA. At Multiplication stage results showed that MS medium supplemented with 2 mg. L-1 of BA with 0.1 mg. L-1 IAA increased numbers of shoot comparing with the Kin; (6.40 shoot /exp.) While the treatment of MS medium supplemented with 0.2 mg. L-1 IAA without BA gave the best shoots length which reached 4.15 cm. In rooting stage, shoots have been cultured in MS medium supplemented with different concentrations of Auxins. Results showed that MS medium at half strength supplemented with 1 mg. L-1 of IBA and 1.5 mg. L-1 IAA significantly increased the number of roots per shoot, roots length up to (7.2 roots/shoot and 6.14 cm) respectively. The survival percentage of plantlets was 70% when they planted in a composed consisted of 3:1 (v:v) peatmoss: soil mixture. We found that BA increased numbers of shoot comparing with the Kin and MS medium at half strength was the best in rooting plantlet comparing with MS medium at full strength.

Response of Strawberry Growth, Yield and Marketable Fruit Quality to Spraying With Moringa Leaf Extract, Calcium and Potassium Silicate

“…Strawberry is among the plants that require high nutrient requirements, as it produces an abundant yield of fruits in proportion to the fruit size. Additionally, the plant is susceptible to diseases and insect infections, and its fruits are non-climacteric, but quickly perishable and have a short shelf life due to the high rate of respiration and high-water content (9,21), Moringa leaf extract is Among the natural plant growth stimulators which is rich in essential nutrients, vitamins, amino acids, and plant growth hormones (14,24), which contributes to providing the nutrients and natural growth regulators required for the plant, reflecting on the yield quantity and quality. Bakhsh et al (8) noted that the foliar application of Moringa leaf extract in four concentrations (0, 2, 4, and 6%) on peach trees; the concentration of 2% had increased plant yield, T.S.S, and vitamin C in the fruits.…”

The Response of Moringa oleifera Lam. Nodes to Multiply on MS and WPM Media Supplemented with Different Concentrations of BA and Kin

“…The most important of which are the type and concentration of cytokinins used in multiplying the plant part and the type of nutrient media and concentration of its components used in the development of the plant part [10]. [11], studied the effect of adding different concentrations of BA and Kin (0, 1, 2, and 4) mg.l -1 to MS medium in the multiplication of the nodes of the Moringa plant. Their results showed that after four weeks of culture, the largest number of shoots reached 2.5 shoots/nodes in the nodes that cultured on the MS equipped with 4.0 mg.l -1 BA compared to 2.20 shoots/nodes resulted from the culture in the medium equipped with 4.0 mg.l -1 Kin, with a significant superiority over the comparison treatment.…”