Gas chromatography with mass spectrometry was used for performing a qualitative analysis of liposoluble flour extract made of different types of small grains (bread wheat, barley and triticale). The results show that the dominant methyl-esters of fatty acids composition is same for all samples, but the percentage of their representation in various types of small grains is different. Transesterification reagent was TMSH (Trimethylsulfonium hydroxide, 0.2M in methanol). With transesterification reaction fatty acids from acilglycerol esterifyed to methyl-esters. In all extract analyzed methyl linoleate predominated, followed by methyl palmitate, methyl oleate and methyl stearate in decreasing order. The following tests cluster analysis was used for the comparison of the liposoluble flour extract different types of small grains

ABSTRACT:In this study, betaine content in cereal grains, cereal-based products, gluten-free grains and products of mainly local origin was surveyed. Estimates of betaine are currently a topic of considerable interest. The principal physiologic role of betaine is as an osmolyte and methyl donor. Inadequate dietary intake of methyl groups causes hypomethylation in many metabolic pathways which leads to alterations in liver metabolism and consequently, may contribute to numerous diseases such as coronary, cerebral, hepatic and vascular. Cereals are the main sources of betaine in human diet. Results showed that betaine content in grains is variable. Spelt grain was found to be a richer source of betaine (1848 g/g DM) than that of common wheat (532 g/g DM). Gluten-free ingredients and products were mainly low in betaine (<150 g/g DM). Amaranth grain is a remarkable gluten-free source of betaine (5215 g/g DM). Beet molasses is an ingredient which may increase betaine content in both cereal-based and gluten-free products.

Extruded amaranth grain products have specific aroma and can be used as snack food, supplement in breakfast cereals, or as raw material for further processing. Extruded products of corn-amaranth grits blends, containing 20% or 50% amaranth grain grits, were produced by extrusion-cooking using a laboratory Brabender single screw extruder 20 DN. Extrudates with various texture were obtained. During extrusion process starch granules are partially degraded, hence rheological properties were examined. All samples exhibited thixotropic flow behavior. Those samples in which part of the corn grits was replaced with amaranth one had lower viscosity and exhibited lower level of structuration during storage

The applicability of Peleg equation was examined for the description of mass transfer during osmotic dehydration (OD) of apple in sugar beet molasses. Mass transfer was investigated in terms of water loss (WL) and solid gain (SG), during OD in 40-80% sugar beet molasses solutions, at 45, 55 and 65ºC. High regression coefficients obtained for Peleg constants (R2>0.975) indicate good fit to the experimental data. The Peleg rate constant varied from 0.144 to 0.785 (g/g i.s.w.) and from 2.006 to 4.436 (g/g i.s.w.) for WL and SG, respectively. The Peleg capacity constant varied from 1.142 to 1.553 (h g/g i.s.w.) and from 8.254 to 11.930 (h g/g i.s.w.) for WL and SG, respectively. The equilibrium WL∞ and SG∞ were estimated using the Peleg model. In addition, the activation energy (Ea) for WL and SG was determined from the relationship between the Peleg rate constant and Arrhenius equation

Article Highlights• Dough and bread quality of substandard flour can be improved by using TG and L-AA • RSM was applied in the analysis of the individual and interactive effect of TG and L-AA • TG has more linear effect in the fermentation process, on the crumb quality than L-AA • L-AA has a much greater effect on the specific volume of bread than TG • Desirability function defines optimum dose of TG and L-AA for sample of flour substandard quality AbstractOver the past decade, extreme variations in climatic conditions have been observed, which in combination with inadequate agro techniques lead to decreased quality of mercantile wheat, i.e. flour. The application of improvers can optimise the quality of substandard wheat flour. This paper focuses on systematic analysis of individual and interaction effects of ascorbic acid and transglutaminase as dough strengthening improvers. The effects were investigated using response surface methodology. Transglutaminase had much higher linear effect on the rheological and fermentative properties of dough from substandard flour than L-ascorbic acid. Both transglutaminase and L-ascorbic acid additions had a significant linear effect on the increase of bread specific volume. Effects of transglutaminase and ascorbic acid are dependent on the applied concentrations and it is necessary to determine the optimal concentration in order to achieve the maximum quality of the dough and bread. Optimal levels of tested improvers were determined using appropriate statistical techniques, which applied the desirability function. It was found that the combination of 30 mg/kg of transglutaminase and 75.8 mg/kg of L-ascorbic acid achieved positive synergistic effects on rheological and fermentative wheat dough properties, as well on textural properties and specific volume of bread made from substandard quality flour.Keywords: substandard quality flour, transglutaminase, L-ascorbic acid, optimization.Over the past decade, an appreciable stagnation of quality and yield of mercantile wheat at the global, regional and local levels has occurred [1][2][3][4]. This phenomenon is the consequence of more

The incidence of mycobiota on common wheat (Triticum aestivum) and spelt (Triticum aestivum ssp. spelta) samples, collected during the harvest in 2015, was investigated. The obtained results showed that more genera of mycobiota were isolated from the common wheat grains than from the spelt grains. The most frequently isolated species from common wheat grains belonged to genus Alternaria (41.7%), followed by Fusarium (15.2%), while the incidence of this mycobiota on the spelt grains were 32.4% and 10.4%, respectively. Aspergillus flavus was identified in 40.0% wheat samples, with the incidence of 2.0%. Additionally, this study was undertaken in respect of the occurrence and toxigenic potential of A. flavus isolates from these small grain cereals. A simple screening method was applied to determine toxigenic profiles (aflatoxins production) of A. flavus isolates from common wheat. The results revealed the importance of precise investigation of mycobiota distribution on common wheat and spelt grains, and especially the investigation of toxigenic potential of A. flavus.

Gas chromatography with mass spectrometry (GC-MS) was used for performing a qualitative analysis of the hexane flour extract of three samples of spelt. All the three samples were first treated with hexane and the obtained extracts were used for the analysis of the fatty acid lipid components. The transesterification reaction was performed using TMSH (trimethylsulfonium hydroxide, 0.2M in methanol), and the fatty acids were esterified from acylglycerol to methyl-esters. In all analyzed extracts, the predominant component was methyl linoleate, followed by methyl oleate and methyl palmitate. The subsequent tests, performed by cluster analysis, were used to compare the hexane flour extracts of different types of spelt. [Projekat Ministarstva nauke Republike Srbije, br. III46005 i br. TR 31066]

This study was conducted to investigate the effect of the addition of buckwheat flour, as well as the influence of buckwheat grain pretreatment, on betaine content in wholegrain wheat pasta. The buckwheat is traditionally used in the human diet for centuries due to its nutritional benefits and positive effect on health such as plasma cholesterol level reduction, anticancer and anti-inflammatory activity. On the other side, betaine is a bioactive compound naturally present in cereals and pseudocereals, important for demethylation of homocysteine to methionine which mainly occurs in the liver. The single screw extruder was used for the production of wholegrain wheat pasta as a control sample and pasta in which wholegrain wheat flour was substituted with one of two types of wholegrain buckwheat flour (autoclaved wholegrain buckwheat flour and flour without treatment), with a level of substitution from 10 to 30%. High-performance liquid chromatography with evaporative light scattering detector (ELSD) was used to analyze betaine content in different pasta samples. Betaine content in wholegrain wheat pasta was 30.07 mg/100 g f.w. (i.e. 34.92 mg/100 g d.w.) and the highest content of betaine in pasta enriched with wholegrain buckwheat flour was detected in a sample with 10% flour substitution (21.63 mg/100 g, apropos 24.22 mg/100 g d.w.) compared to other levels of substitution. Following the presented results, autoclaving pretreatment of buckwheat grain prior to buckwheat flour production did not show important differences in betaine content when pasta made with this type of flour was compared with pasta made with untreated buckwheat flour.