Performance of GC-MS analysis for differentiation of various types of flour by creating dendrogram of liposoluble extract

Abstract

Gas chromatography with mass spectrometry was used for performing a qualitative analysis of liposoluble flour extract made of different types of small grains (bread wheat, barley and triticale). The results show that the dominant methyl-esters of fatty acids composition is same for all samples, but the percentage of their representation in various types of small grains is different. Transesterification reagent was TMSH (Trimethylsulfonium hydroxide, 0.2M in methanol). With transesterification reaction fatty acids from acilglycerol esterifyed to methyl-esters. In all extract analyzed methyl linoleate predominated, followed by methyl palmitate, methyl oleate and methyl stearate in decreasing order. The following tests cluster analysis was used for the comparison of the liposoluble flour extract different types of small grains

Red Algae (Rhodophyta) from the Coast of Madagascar: Preliminary Bioactivity Studies and Isolation of Natural Products

“…Purification of the dichloromethane extract by column chromatography using pentane-diethyl ether (7:3) as eluent led to the isolation of a mixture of cholesterol ( 5 ) and 24-methyl-25-homocholesterol ( 8 ) (Figure 3). Compound 8 has been mentioned several times in the literature but to the best of our knowledge only as a hit during a library search from a GC-MS database [21,22,23,24,25]. In the present work, we describe the NMR data of 8 by complete assignment of all 1 H NMR and 13 C NMR signals of the mixture with cholesterol ( 5 ) using COSY, NOESY, HMBC, and HSQC measurements.…”

Homogenity of oil and sugar components of flour amaranth investigated by GC-MS

“…Figure 2 shows two areas with silyl derivatives of carbohydrates. The results of the chromatogram The carbohydrate content was much lower than the unbound triglyceride content [17], and therefore the remains of triglycerides are higher than the content of carbohydrates.…”

“…flour by creating the dendrograms of liposoluble and hydrosoluble extracts. Such studies have been conducted on wheat [17,18], spelt [19,20] and buckwheat [submitted for publication]. It was detected that each plant species has a characteristic composition of liposoluble and hydrosoluble extracts.…”

Ratio of omega-6/omega-3 fatty acids of spelt and flaxseed pasta and consumer acceptability

“…Samples were prepared as described by Vujić et al (2012); trimethylsulfonium hydroxide (TMSH), 0.2 M in methanol, was used as a derivatisation reagent; temperature programs were: 50-130°C at 30°C/min and 130-300°C at 10°C/min; injector temperature was 250°C; the flow of the carrier gas (helium) was 0.8 ml/min, split ratio of 1 : 50 was used for the injection of 1 μl of dissolved sample.…”