The bovine coronavirus strain LY-138 was purified by differential as well as velocity and isopycnic centrifugation in sucrose or CsCl gradients. The substrate for purification was contents of the small intestine of experimentally inoculated calves. This strain is highly enteropathogenic, but it could not yet be propagated in cultured cells. Intact virions had a density of 1.245 g/cm3 in CsCl and 1.185 g/cm3 in sucrose. A spherical core-like structure with an average diameter of 82 nm remaining after treatment with chloroform had a density of 1.299 g/cm3 in CsCl and 1.201 g/cm3 in sucrose. Seven distinct bands of polypeptides and 4 shoulders were detected after electrophoresis of SDS-solubilized virions in polyacrylamide gels. The approximate molecular weights ranged from 110,000 to 36,000. Four of the bands gave a PAS positive reaction. These 4 glycoproteins and an additional protein with an approximate molecular weight of 70,000 were removed by chloroform treatment. The remaining core-like structure contained the 2 polypeptides VP3 and VP7.
L'étude tente de clarifier le rôle des bovins apparemment sains comme réservoir du virus de la fièvre catarrhale à l'égard des moutons au Soudan. Elle confirme les travaux antérieurs et établit que les bovins peuvent héberger le virus auquel sont sensibles les ovins de ce pays. Les conditions de transmission expérimentale du virus entre les deux espèces suggèrent que le meilleur indicateur pour déterminer la virémie sur des bovins apparemme nt sains consiste à inoculer des moutons sensibles avec le virus bovin suspecté. Les conditions de la virémie et la survie du virus dans la nature font l'objet d'une discussion.
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