Three minor indole alkaloids have been isolated from "hairy roots" of Rauwolfia serpentina Benth.: 3-epi-α-yohimbine (1), 18β-hydroxy-3-epi-α-yohimbine (2), and 12-hydroxyajmaline (3). The latter compound (3) is a novel alkaloid. The alkaloid structures have been determined by spectroscopic data.
Peganum harmala, Hairy Root Cultures, β-Carboline Alkaloids Hairy root cultures of Peganum harmala were established by genetic transformation with Agrobacterium rhizogenes strain A4. In contrast to suspension cultures the root cultures contained high levels of β-carboline alka loids (1 -1.5% of dry mass).
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