Alkaloid Formation in Hairy Roots and Cell Suspensions of<i>Rauwolfia serpentina</i>Benth

Falkenhagen, Heike; Кузовкина, И. Н.; Alterman, I. E.; Nikolaeva, L.; Stöckigt, Joachim

doi:10.1080/10575639308043846

Cited by 27 publications

(18 citation statements)

References 7 publications

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“…Hairy root cultures have been established from important medicinal plants in the Apocynaceae family e.g. C. roseus (Toivonen et al 1989;Bhadra et al 1993), R. serpentina (Falkenhagen et al 1993;Benjamin et al 1993) and Rauvolfia micrantha (Sudha et al 2003). In the present study, an easy and convenient method for establishing transgenic hairy roots of R. stricta through infection with wild-type and transformed A. rhizogenes LBA 9402 was developed for the first time.…”

Section: Discussionmentioning

confidence: 99%

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

et al. 2015

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Transgenic hairy roots of R. stricta were developed for investigation of alkaloid accumulations. The contents of five identified alkaloids, including serpentine as a new compound, increased compared to non-transformed roots. Rhazya stricta Decne. is a rich source of pharmacologically active terpenoid indole alkaloids (TIAs). In order to study TIA production and enable metabolic engineering, we established hairy root cultures of R. stricta by co-cultivating cotyledon, hypocotyl, leaf, and shoot explants with wild-type Agrobacterium rhizogenes strain LBA 9402 and A. rhizogenes carrying the pK2WG7-gusA binary vector. Hairy roots initiated from the leaf explants 2 to 8 weeks. Transformation was confirmed by polymerase chain reaction and in case of GUS clones with GUS staining assay. Transformation efficiency was 74 and 83% for wild-type and GUS hairy root clones, respectively. Alkaloid accumulation was monitored by HPLC, and identification was achieved by UPLC-MS analysis. The influence of light (16 h photoperiod versus total darkness) and media composition (modified Gamborg B5 medium versus Woody Plant Medium) on the production of TIAs were investigated. Compared to non-transformed roots, wild-type hairy roots accumulated significantly higher amounts of five alkaloids. GUS hairy roots contained higher amounts two of alkaloids compared to non-transformed roots. Light conditions had a marked effect on the accumulation of five alkaloids whereas the composition of media only affected the accumulation of two alkaloids. By successfully establishing R. stricta hairy root clones, the potential of transgenic hairy root systems in modulating TIA production was confirmed.

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Section: Discussionmentioning

confidence: 99%

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

et al. 2015

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“…A fter scaling-up such an incubation 20-fold, GC-MS analysis of the acetylated assay mixture showed the enzymatic formation of vomilenine unequivocally by the characteristic fragmentation pattern of its acetylated derivative (Falkenhagen et a l, 1993).…”

Section: Resultsmentioning

confidence: 99%

“…All other commercially available m aterials were of highest purity. Vinorine and vomilenine were isolated from R. serpentina hairy root cultures in our laboratory (Falkenhagen et al, 1993).…”

Section: Materials and M Ethodsmentioning

confidence: 99%

Enzymatic Biosynthesis of Vomilenine, a Key Intermediate of the Ajmaline Pathway, Catalyzed by a Novel Cytochrome P 450-Dependent Enzyme from Plant Cell Cultures of Rauwolfia serpentina

Falkenhagen

Stöckigt

1995

Zeitschrift Für Naturforschung C

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“…Different stationary phases were used in the past for normal phase packing, e.g. aluminum oxide [25]and silica gel [26], or reversed-phase materials like RP8 [27] or RP18 [28,29].…”

Section: Quantitative and Qualitative Analysis Of Indolealkaloidsmentioning

confidence: 99%

Physiological, Biochemical and Modern Biotechnological Approach to Improvement of Rauwolfia serpentina

Poonam¹

2013

IOSR-JPBS

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Alkaloid Formation in Hairy Roots and Cell Suspensions ofRauwolfia serpentinaBenth

Cited by 27 publications

References 7 publications

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

Enzymatic Biosynthesis of Vomilenine, a Key Intermediate of the Ajmaline Pathway, Catalyzed by a Novel Cytochrome P 450-Dependent Enzyme from Plant Cell Cultures of Rauwolfia serpentina

Physiological, Biochemical and Modern Biotechnological Approach to Improvement of Rauwolfia serpentina

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