Genetically Transformed Plant Roots as a Model for Studying Specific Metabolism and Symbiotic Contacts of the Root System

Aseptic Germination and Agrobacterium rhizogenesmediated Transformation of Taraxacum hybernum Steven

“…The rol genes of Agrobacterium rhizogenes can be transferred to the culture of isolated roots for making their growth more active. Expression of rol genes promotes rapid and unlimited growth of isolated root cultures on hormone-free media (Kuzovkina et al 2004). Therefore, there is a need to develop protocols for the genetic transformation of T. hybernum for applied research, such as metabolic engineering.…”

Влияние Синтетического Антиоксиданта Тиофана И Экстракта Трансформированных Корней Шлемника Байкальского На Индуцированный Мутагенез

“…Òèîôàí äîáàâëÿëè â ïèòàòåëüíóþ ñðåäó â êîíöåíòðàöèè 0,056 % â 1 % êðàõìàëüíîé ñëèçè.  êà÷åñòâå êîððåêòîðà ðàñòèòåëüíîãî ïðîèñõîaeäåíèÿ èñïîëüçîâàëè ÝØÁ (È. Í. Êóçîâêèíà, Èíñòèòóò ôèçèîëîãèè ðàñòå-íèé ÐÀÍ, Ìîñêâà) [14]. ÝØÁ ïðåäñòàâëÿåò ñîáîé êîìïëåêñ âåùåñòâ, èçâëåêàåìûõ èç ñûðüÿ 70 % ðàñòâîðîì ýòàíîëà, è ñîäåðaeèò 72 % âîãîíèçèäà è âîãîíèíà (63,3 è 8,6 %, ñîîòâåòñòâåííî) îò îáùåãî ñîäåðaeàíèÿ ôëàâîíîâ.…”

“…Ïðèìåíåíèå äàííîãî ñðåäñòâà îáîñíîâàíî åãî ðàíåå ïîêàçàííûì øèðîêèì ñïåêòðîì áèîëîãè÷åñêèõ ýôôåêòîâ (èììóíîñòèìóëèðóþùåå, ãåìàòîñòèìóëèðóþùåå, ìåìáðàíîòðîïíîå, àíòèîêñèäàíòíîå è äð.) [12,14].  êà÷åñòâå êîíòðîëÿ èñïîëüçîâàëè äèñòèëëèðîâàííóþ âîäó.…”

“…Óìåíüøåíèå êîëè÷åñòâà ìîçàè÷íûõ îñîáåé ìîaeíî îáúÿñíèòü ñíèaeåíèåì èíòåíñèâíîñòè îáìåíîâ ãîìîëîãàìè õðîìîñîì, ÷òî, â ñâîþ î÷åðåäü, çàâèñèò îò ÷àñòîòû ðàçðûâîâ ýòèõ ãåíåòè÷åñêèõ ñòðóêòóð. Ñíèaeåíèå ÷àñòîòû ðàçðûâîâ õðîìîñîì îáóñëîâëåíî ñïîñîáíî- ñòüþ êîððåêòîðîâ èíãèáèðîâàòü ïðîöåññû ïåðåêèñíîãî îêèñëåíèÿ ëèïèäîâ, â ñâÿçè ñ ÷åì óìåíüøàåòñÿ ïîâðåaeäàþùåå äåéñòâèå àêòèâíûõ ðàäèêàëîâ íà ãåíåòè-÷åñêèå ñòðóêòóðû [6,12,14]. Èñïîëüçîâàíèå òèîôàíà è ÝØÁ ñïîñîáñòâîâàëî ñîõðàíåíèþ öåëîñòíîñòè õðîìîñîì è, ñîîòâåòñòâåííî, ñíèaeàëî âåðîÿòíîñòü îáìåíà èõ ôðàãìåíòàìè.…”