The difference in optimal conditions for DNA polymerization catalyzed by AMV reverse transcriptase on poly(A) and poly(dA) templates with d(pT)~ o and (pU)~ o primers has been found. A comparison of the initiating abilities of d(pT)~ o and (pU)l o primers under optimal conditions for various template, primer complexes has been made. The best template, primer complex was poly(A), d(pT)lo and the worst was poly(A). (PU)lo. The lengthening of d(pT)~ primers by a mononucleotide unit (n = 2-10) increases their affinity by a factor of about 2 and 3 in the case of poly (dA) and poly(A) templates, respectively. The affinities of d(pT)H_25 to the enzyme does not change with the primer length.
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