A comparison of the initiating abilities of ribo‐ and deoxyriboprimers in DNA polymerization catalyzed by AMV reverse transcriptase

Lokhova, I A; Nevinsky, Georgy A.; Gorn, V. V.; Веньяминова, А. Г.; Repkova, M V; Kavsan, V. M.; Rudenko, N.K.; Lavrik, Olga I.

doi:10.1016/0014-5793(90)81352-o

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1993

2005

Publication Types

Select...

Article4

Book1

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 8 publications

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Comparison of interactions of 5?-derivatives of deoxyoctathymidylate with human DNA polymerize ? and HIV reverse transcriptase

et al. 1993

View full text Add to dashboard Cite

Km and Vmax values for d(pT8) and its derivatives containing various 5'-end groups were estimated in the reaction of DNA polymerization alpha catalyzed by DNA polymerase alpha and HIV-RT. The effect of 5'-end modification of primer is more pronounced in the case of HIV-RT. Strong influence is observed for an intercalating (ethidium) group. The affinity of EtpT8 is 200-fold higher than that of d(pT8). Attachment of Phn-, Dnm- and Hem-groups results in the increase of affinity of modified primer from 10 up to 20 times. For DNA polymerase alpha the influence of modifiers on primer affinity is much weaker. The effect of 5'-end residues on the Vmax values is also more pronounced for HIV RT. The way to improve selective interaction of oligonucleotide derivatives with the primer site of HIV RT is suggested.

show abstract