Pasteurella multocida (46.0%), Neisseria spp. (44.0%), Aspergillus fumigatus (10.0%) were isolated in stable consortia form from milk samples (n = 60) from cows in 6 herds (n = 627 heads) with clinical signs of immunodeficiency status. The bacteria of these consortia is strong resistant to lots of antibiotics from synthetic penicillin group (ampiox, amoxiclav, ampicillinum), cephalosporins (cefalexin, cefixime), and macrolides (macropene, tylosin). The etiological structure of bacterial infections in cattle and their calves (n = 57) in mentioned farm holdings of eastern Ukraine was determined (6 farms in 3 regions). In the majority of these livestock, respiratory syndrome has been recorded for a long time in 56.0% of calves from 20 days to 5 months old. The signs of diarrhea were revealed in 12.0% of lactating and dry cows of these livestock, 18.0% of these animals showed the clinical manifestation of mastitis and 9.0% of them had the diarrhea syndrome. Pasteurella multocida (48.0%), Mycoplasma bovis (32.0%), Neisseria spp. (12.0%), Clostridium perfringens (8.0%) were identified using microbiological methods from nasal and rectal swabs from calves of different age groups, from cows with clinical signs of mastitis were isolated Mycoplasma bovis (50.0%), Pasteurella multocida (34.0%), Neisseria spp. (16.0%). Pasteurella multocida, Neisseria spp. and Clostridium perfringens isolates showed strong resistance to ampiox, amoxiclav, ampicillinum, cefalexin, cefixime, macropene, and tylosin. It was concluded that mentioned multidrug-resistant animal infectious agents in human food chain could be a sources of antibiotic resistance genes for human pathogens.
The article presents data on the optimization of the clinical protocol for the use of oil and adsorbed forms of experimental vaccine “ReproSuiVac-P” in industrial pig breeding (in the nucleus of herd, in groups of growing and fattening) in the system of biosafety measures “StopASFmix” based on immunological indicators of immunity, including humoral and cellular, with regards of the average daily growth of piglets) and specific (dynamics of postvaccinal antibodies, the intensity of population immunity, the resistance of vaccinated pigs relative to carrier-status of Mannheimia haemolytica). The obtained results allowed, at the level of probability not less than p≤0.01 (n = 879 pigs of different technological groups), to develop a technological card of vaccinations, which provides application of: a) oil form “ReproSuiVac-P” with bacterin on boars, lactating and barren sows twice with an interval 3 weeks in doses of 5+5 sm3; b) oil form of the vaccine in the group of weaned piglets 32nd–35th days of age at a single dose of 1–2 sm3; c) adsorbed with toxoid form of vaccine to sows of the second trimester of gestation twice with an interval of 2 weeks in doses of 5+7 sm3. Based on the indicators of constitutional and specific post-vaccination immunity against associated circovirus-pasteurellosis infection, a technological card of application of experimental vaccine “ReproSuiVac-P” in the main herd and in rearing groups of the industrial pig breeding was developed
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