No abstract

Periosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). SH2(+), SH3(+), SH4(+), CD9(+), CD90(+) and CD105(+) were important eternal characteristic cell surface markers for the PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures until the 15th passage from primary cell culture.

A general synthetic route to the set of polyhydroxylated agarofurans that comprise the core structures of esters present in plants of the Celastraceae family has been devised. The pathway is exemplified with total syntheses of (±)-3,4-dideoxymaytol (3), the nucleus of ever-1 (6), and (±)-euonyminol (4), the sesquiterpenoid core of several cathedulins including K-19 (5). A focal intermediate 19, prepared by Diels−Alder addition of 11 to 12, was identified that permitted stereoselective introduction of an isopropenyl substituent via chelation-controlled, conjugate Grignard addition to give 34. Triflic acid-catalyzed cyclization of 39 afforded the agarofuran skeleton of 3, and subsequent epimerization of the hydroxyl substituent at C1 via a reversible aldol sequence gave 41. Sequential reductions using hydride and catalytic hydrogenation yielded 3 and 4-epi-3,4-dideoxymaytol (51). The route from 19 was extended toward 4 via 56, prepared by directed epoxidation of 34. A trifuoroacetic acid-catalyzed “epoxide-cascade” cyclization of 56 furnished 61, which was advanced to γ-lactone 63 prior to epimerization at C1. Introduction of the 8β hydroxyl function of 69 was followed by an α-ketol transposition to give 71 which was reduced and protected as polysilyl ether 80. Osmylation and replacement of protecting groups produced euonyminol octaacetate 78 which underwent methanolysis to (±)-4.

Nanopatterned 2-dimensional Au nanocluster arrays with controlled configuration are fabricated onto reconstructed nanoporous poly(styrene-block-vinylpyridine) inverse micelle monolayer films. Near-field coupling of localized surface plasmons is studied and compared for disordered and ordered core-centered Au NC arrays. Differences in evolution of the absorption band and field enhancement upon Au nanoparticle adsorption are shown. The experimental results are found to be in good agreement with theoretical studies based on the finite-difference time-domain method and rigorous coupled-wave analysis. The realized Au nanopatterns are exploited as substrates for surface-enhanced Raman scattering and integrated into Kretschmann-type SPR sensors, based on which unprecedented SPR-coupling-type sensors are demonstrated.

The dependence of band intensities in the Raman spectrum of individual single-crystal ruthenium dioxide (RuO2) nanowires on the angle between the plane of polarization of the exciting (and collected) light and the long axis of the nanowire, is shown to be a simple, complementary technique to high resolution transmission electron microscopy (HRTEM) for determining nanowire growth direction. We show that excellent agreement exists between what is observed and what is predicted for the polarization angle dependence of the intensities of the nanowires’ Eg (525 cm−1) and the B2g (714 cm−1) Raman bands, only by assuming that the nanowires grow along the (001) crystallographic direction, as confirmed by HRTEM.